Microevolution: Unique Gene Pools

1. Microevolution: Unique Gene Pools

2. Charles Darwin Charles Darwin (1809-1882) is credited with proposing that the mechanism for the process of evolution is natural selection. Darwin spent five years on a voyage that took him around the world with the majority of his time spent in South America and its neighboring islands. Darwin published his theory with compelling evidence for evolution in his 1859 book On the Origin of Species, overcoming scientific rejection of earlier concepts of transmutation of species.

3. Charles Darwin He established that all species of life have descended over time from common ancestors, and proposed the scientific theory that this branching pattern of evolution resulted from a process that he called natural selection, in which the struggle for existence has a similar effect to the artificial selection involved in selective breeding.

4. Charles Darwin By the 1870s the scientific community and much of the general public had accepted evolution as a fact. However, many favored competing explanations and it was not until the emergence of the modern evolutionary synthesis from the 1930s to the 1950s that a broad consensus developed in which natural selection was the basic mechanism of evolution. In modified form, Darwin's scientific discovery is the unifying theory of the life sciences, explaining the diversity of life.

5. Darwin’s Observations • There is variation within a given species and the majority of this variation is inherited. This litter of kittens vary with respect to coat pattern and color. • Any variation may, to some degree, affect the ability of an organism to reproduce and contribute genes to the gene pool, thus affecting evolutionary success. • Species change over time. These changes are related to traits that are inherited or arise from an alteration of the genetic code. • Some inherited traits are beneficial and contribute to survival. • Whether a trait is beneficial or not is a function of the environment in which it lives.

6. Adaptations and Fitness • An adaptation is a genetically controlled trait that is favored by natural selection and gives the organism a reproductive advantage ensuring the trait is passed on to its descendants. • This trait may also allow the individual to survive longer thus increasing the reproductive rate of that individual.

7. Adaptations and Fitness • The antelope hare lives in the desert, and the snowshoe hare lives in the mountains. • Explain how the differences in their traits enhance their ability to survive in their respective environments. • Evolutionary success or fitness refers to the contribution of genes to the gene pool and NOT how long an organism lives.

8. The Effect of Environmental Change Earth’s environment is NOT STATIC, but rather ever changing. Environmental changes often cause a shift in selection pressures. As a consequence, traits or adaptations that were favorable may become unfavorable. The peppered moth, Bistonbetularia is native to England and exists in two forms, one is dark and the other light with a “peppered” appearance. Birds are its main predator. Prior to the industrial revolution, only 2% of the moths were dark. The industrial revolution produced vast amounts of sulfur dioxide and soot from the burning of coal which altered the environment.

9. Figure it out with a partner!! Propose an explanation why fifty years later 95% of the moths were dark.

10. Industrial Melanism England has since regulated the burning of coal and as a result, the trees are returning to their original state (A). Consequently, the coloring among the population of moths in Britain has shifted back so that the peppered moths are once again favored.

11. Evolution Defined • Evolution is defined as a change in the inherited characteristics of biological populations over successive generations. • Evolutionary processes give rise to diversity at every level of biological organization, from the molecular to the macroscopic. • As a result, diversity is prevalent among molecules such as DNA as well as individual organisms and species of organisms.

12. Microevolution Microevolution is simply a change in gene frequency within a population. • Evolution at this scale can be observed over short periods of time such as from one generation to the next. • Example: The frequency of a gene for pesticide resistance in a population of crop pests increases. • Such a change might come about because • natural selection favored the gene • the population received new immigrants carrying the gene (gene flow) • nonresistant genes mutated into a resistant version of the gene • of random genetic drift from one generation to the next

13. Microevolution • A gene is a sequence of DNA nucleotides that specify a particular polypeptide chain. • Genes code for proteins. • An allele is a particular form of a gene. For example: B represents the allele for black coat color and b for white coat color. • Selection acts on phenotype because differential reproduction and survivorship depend on phenotype not genotype. Explain. • Natural selection acts on individuals, but only populations evolve.

14. Figure it out with a partner!! • Selection acts on phenotype. • Explain why differential reproduction and survivorship depend on phenotype not genotype.

15. Figure it out with a partner!! • Natural selection acts on individuals. • Explain why only populations evolve and NOT individuals.

16. Macroevolution Macroevolution is evolution on a scale of separated gene pools (not individuals). • Think of it as an accumulation of changes which result in speciation (forming a new species). • Macroevolutionary studies focus on change that occurs at or above the level of species. • Macroevolution can explain: • the evolution of more complicated things like the how the eye evolved • how speciation occurs

17. More Evolution Terms • Species-a group of interbreeding organisms that produce viable and fertile offspring in nature • Gene pool- total of all the genes in a given species • Allelic frequency-is the percent occurrence for a given allele

18. Figure it out with a partner!! Use the image to explain the difference between allele frequency and gene pool.

19. Sources of Genetic Variation How does variation in a population or gene pool arise? Mutations, gene duplication and chromosome fusion provide the raw material for evolution. Meiosis and sexual reproduction produce new recombinants of phenotypes upon which natural selection operates. The wisteria pictured on the right has a mutation causing it to produce white flowers instead of purple flowers.

20. Types of Mutations • MOST mutations are recessive and deleterious (fatal). • Obviously, mutations occurring in somatic cells do not affect future generations. • Only mutations occurring in gametes affect future generations. • Mutations can occur at either the gene or chromosomal level. Mutations may cause a sheep to have a 5th leg. But this is not evolution!

21. Point Mutations: Synonymous vs. Nonsynonymous Point mutations occur when one nucleotide is substituted for another. The genetic code contains “synonyms” for the coding of amino acids. For example the DNA codons GGA, GGG, GGT, GGC all code for the amino acid proline. Therefore, as long as the codon has GG in positions 1 & 2, a mutation in position three has no consequence, proline will be coded for regardless. This sort of mutation is called a synonymous or silent mutation.

22. Point Mutations: Synonymous vs. Nonsynonymous Point mutations that do result in a different amino acid are called a nonsynonymous or missense mutations. Missense mutations can affect the protein in one of THREE ways: (Remember the new amino acid will have a different R group on the protein) It can result in a protein that does not function as well as the original protein. (This happens most often.) It can result in a protein that functions better than the original protein. It can result in a protein that functions like the original protein. This is usually because the R groups are similar. (both polar or both nonpolar, etc.)

23. Point Mutations: Synonymous vs. Nonsynonymous Another type of nonsynonymous mutation is referred to as a nonsense mutation. In a nonsense mutation, the altered nucleotide sequence results in a premature stop codon which produces a truncated, incomplete and usually nonfunctional protein.

24. Gene Duplication Genes can be duplicated and occasionally the duplication moves a gene from one chromosome to another. Each gene will accumulate different mutations altering the protein that is subsequently synthesized. Myoglobin is a protein that binds with oxygen in the muscles. This gene has been duplicated and modified many times. Many of the duplications are introns – noncoding segments of DNA. While some of the modified genes are exons which code for functional proteins. It has given rise to the hemoglobin gene.

25. Neutral Mutations • Naturally evolving proteins gradually accumulate mutations while continuing to fold into stable structures. This process of neutral evolution is an important mode of genetic change and forms the basis for the molecular clock. • Cytochromec is a small protein found on the mitochondrial membrane. • Between mammals and reptiles there are 15 different amino acids or mutations.

26. Neutral Mutations • Mammals and reptiles diverged 265 million years ago. • That means on average cytochromec mutated every 17 million years. • In comparing the evolution of other organisms and their cytochromec one mutation every 17 million years holds true.

27. Changes in Cytochrome C Above is a comparison ancestral cytochrome c and human cytochrome c. This gene has been highly conserved as it is a protein used in the electron transport chain of the mitochondria. Missense mutations occur more frequently in pseudogenes (genes that have been duplicated, then mutated and are no longer functional) than in functional genes.

28. Cytochrome c Comparison • A dash indicates that the amino acid is the same one found at that position in the human molecule. • All the vertebrate cytochromes (the first four) start with glycine (Gly). • The Drosophila, wheat, and yeast cytochromes have several amino acids that precede the sequence shown here (indicated by <<<). • In every case, the heme group of the cytochrome is attached to Cys-14 and Cys-17 (human numbering).

29. Hemoglobin Comparison • This is a comparison between the differences in the amino acid sequence of human hemoglobin and different species. • The last three species do not have a distinction between a and b chains. • There is an inverse relationship between the difference in the amino acid sequence and how closely related the organisms are to humans. • The b chain of hemoglobin has 146 amino acids.

30. Hemoglobin Comparison

31. Evolution of Hemoglobin Gene

32. Frameshift Mutation • A frameshift mutation occurs as a result of either an insertion or deletion of a nucleotide. • This changes the amino acid sequence of the protein from that point forward. • Almost all frame shift mutations are deleterious. • Recently, bacteria were found growing in a pool of nylon wastes. (Flavobacterium) • These bacteria were actually digesting the nylon waste. • Upon examining the genome of these bacteria, it was found there was a frameshift mutation in their DNA that caused the production of three different enzymes that could digest the nylon.

33. Chromosomal Rearrangement • There have also been major changes in chromosome structure that result in changes within populations which can, in turn, result in the emergence of new species. • These include: • inversions • deletions • duplication • translocations • fusions

34. Chromosomal Rearrangement Compare the karyotype of a human (H) and a chimpanzee (C). Notice the great apes have 24 pairs of chromosomes compared to 23 pairs of chromosomes in a human. Why the difference? Chromosome #2 in the human is the result of a fusion of two chimpanzee chromosomes.

35. Human Impact on Gene Pools It is well documented that humans have had an impact on certain gene pools. For example, humans have selected for certain desirable traits within the mustard family and cultivated different agricultural products for human consumption.

36. Artificial Selection When humans manipulate a gene pool it is called artificial selection. There are often consequences involved in such manipulations. For example in agriculture, farmers try to increase crop production, which may lead to many farmers growing only one variety of a particular crop such as corn. This leads to a loss of genetic diversity. If a disease attacks that particular variety of corn, the farmers growing that variety lose their entire crop.

37. Antibiotics and Artificial Selection • When antibiotics are applied to a population of microorganisms to treat an infection, some of the microorganisms may be naturally immune to the drug. • Why? A random mutation occurred in the genetic code of the microorganism conferring its resistance. • These resistant microorganisms continue to flourish and cause disease. • The only remaining option a physician has is to treat the infection with a different antibiotic and hope that none of the surviving microorganisms possess a different random mutation that makes them resistant to the second antibiotic as well.

38. Antibiotics and Artificial Selection • The increase in antibiotic-resistant bacteria has caused doctors to reduce the number of prescriptions written for antibiotics in general. • About 70% of pathogenic bacteria are resistant to at least one antibiotic and are called “super bugs” or MDR bacteria. (multidrug resistant)

39. MRSA or Methicillin-resistant Staphylococcus aureus • MDR bacteria do not respond to “first line of defense” antibiotics. • These types of bacteria are most commonly found in hospitals. • Skin boils or similar lesions that do not heal often result. • MDR bacteria can attack internal organs upon gaining entry into the body.

40. Reducing or Eliminating Gene Pools • Human activities often augment genetic drift and diminish gene flow for many species. • This reduces genetic variation thereby disrupting adaptive processes both locally and globally within a species. • This impact is illustrated within populations of collared lizards (Crotaphytus collaris) living in the Missouri Ozarks. • Forest fire suppression has reduced habitat and disrupted gene flow in this lizard, thereby altering the balance toward drift and away from gene flow. This balance can be restored by managed landscape burns.

41. Effect of Sexual Reproduction Sexual reproduction recombines genes in new ways. This results in unique offspring that differ from either parent or sibling. Humans make 223 different kinds of gametes. Fertilization means that the uniqueness of an individual is 223 223. Or the probability that two siblings will be genetically identical (excluding identical twins) is 446. Sexual reproduction is like shuffling a deck of cards and every time getting a new and unique hand dealt. It is the major driving force of evolution.

42. Created by: Carol Leibl Science Content Director National Math and Science