1 / 18

Chiral Technologies Development Ltd. Rumbach S. 7., Budapest, H-1075, Hungary

Chiral Technologies Development Ltd. Rumbach S. 7., Budapest, H-1075, Hungary Phone: 36-1-352-2405 Fax: 36-1-413-6944 E-mail: chiroquest@chello.hu web: www.chiroquest.org. Chemically bonded 4-nitrophenyl-urea stationary phases. π-Select high surface coverage fully endcapped.

wilda
Download Presentation

Chiral Technologies Development Ltd. Rumbach S. 7., Budapest, H-1075, Hungary

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Chiral Technologies Development Ltd. Rumbach S. 7., Budapest, H-1075, Hungary Phone: 36-1-352-2405 Fax: 36-1-413-6944 E-mail: chiroquest@chello.hu web: www.chiroquest.org

  2. Chemically bonded 4-nitrophenyl-urea stationary phases • π-Select • high surface coverage • fully endcapped • CD-Screen • optimized coverage, according to the size of cyclodextrin molecule • free silanols H-acceptor δ+ H-donors hydrophylic hydrophobic

  3. DIMETHYL-PHTALATE click on image for animation

  4. CAFFEINE click on image for animation

  5. PHENOL click on image for animation

  6. Polar analytes π-Select k’=0.54 Conventional phenyl-column k’=0.25 Eluent: MeOH-water 30:70 1.: Phenol 2.: Caffeine

  7. Apolar, aromatic analytes π-Select C: 4.7% Conventional phenyl-column C: 7.0% Eluent: MeOH-water 60:40 1.: Dimethyl-phtalate; 2.: Diethyl-phtalate 3.: Biphenyl 4.: o-Terphenyl

  8. Diastereomer peptides * Tyr-Pro-Phe-Atc-NH2 Eluent: 0.1% TFA in water-0.1% TFA in MeOH 75:25 Atc-NH2: 2-amino-decaline-2-carboxylicacid * : racemic amino acid Basic drugs Eluent: MeOH-0.1% H3PO4 60:40 0.8 ml/min pH: 2.6 1.: N-methyl-ephedrine; 2.: Athenolol 3.: Pindolol; 4.: Sertraline

  9. 1. Benzothiophene 6. Benzo[a]pyrene 3 2 4 2 1 1 7. Benzo[g,h,i]perylene 3 2. Fluorene 7 5 5. Pyrene 6 3. Fluoranthene 4. Dibenzothiophene Separation of Polinuclear Aromatic Hydrocarbons on Pi-Select column methanol-water 85:15 methanol-water 75:25

  10. π-Select Plates: 16685 Asymmetry: 1,189 cis and trans R and S LiChrosphere Si 60 Plates : 5649 Asymmetry : 1,261 Prostaglandine intermediate R * Eluent: n-hexane - TBME 97:3

  11. 1.: dehydroepiandrosterone 2.: testosterone 3.: androstenedione 4.: pregnenolone 5.: dehydroepiandrosterone-sulfate Steroids in blood Juvenile hormone (insecticide) geometrical isomers

  12. CD-SCREEN Inclusion complex with beta-cyclodextrin

  13. click on image for animation

  14. Hydroxypropyl-β-CD the most likely candidate for incorporation into various drug formulations • Nucleosil 120-7 NH2 column, normal phase • solubility problems • β-CD contamination is the last peak, the integration is difficult • poor resolution of isomeric groups • CD-Screen column, reversed phase • good solubility in the eluent • β-CD contamination is the first peak, high accuracy analysis is possible • detailed „fingerprint” chromatogram . β-CD β-CD

  15. USP method for analysis of Hydroxypropylbetadex TNP: 1800/m 60 min. Alternative method on CD-Screen column TNP: 32000/m 20 min.

  16. Randomly methylated β-CD Degradation products

  17. Randomly methylated α-CD α-CD

  18. -Select • Diametrically Different Retention Mechanism • Unique Selectivity • Good alternative when current C18 fails to deliver • Diametrically different selectivity to other reversed phase columns • Embedded H-donor and H-acceptor groups • High retention of aromatics due to - interactions • Excellent shape-selectivity • 100% water can be used as eluent, no collapse • High polarity reversed phase • A single column can be used in reversed and normal phase separations • Based on “B”-type, High Purity Silica, fully endcapped • Available with 3m and 5m particle size for analytical purpose, and 10m particle size for preparative applications. • For more information please visit our web pages: www.chiroquest.org

More Related