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Microworlds Project

Microworlds Project. Students, please get your microscopes out for today’s lesson. Find your scope number from the green tag posted at your desk. Always carry with 2 hands! 10 Microworlds are required this term (5 pages) –Forms on my website & the lab manual.

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Microworlds Project

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  1. Microworlds Project • Students, please get your microscopes out for today’s lesson. Find your scope number from the green tag posted at your desk. Always carry with 2 hands! • 10 Microworlds are required this term (5 pages) –Forms on my website & the lab manual. • First Microworld due at the end of today! Samples in today’s lab that you may use include: human cheek cells & bacteria slides. • Other samples include pond water and the Microworld prepared slides.

  2. Microscope Care • Always carry with 2 hands & keep scopes away from edges of tables. • Do not force knobs (100X & 40X will break the slides! Do not use course focus w/ 100x & 40x!) • Only use lens paper & lens cleanerfor cleaning. • Handle cords appropriately!! • Put away as listed oncabinet door.

  3. Microscope Parts Eyepiece (ocular lens) Body Tube Revolving nosepiece Arm Objective Lenses Stage Stage/slide holder Coarse Focus Condenser & Iris Diaphragm Fine Focus Light (lamp) Base Animation: Starr Ch 4 How a Microscope Works

  4. Using the Microscope • (JV, walk students thru microscope) • Put the stage all the way DOWN • Place a Slide on the Microscope • Rotate to the lowest (shortest) objective power (it should be stored this way) • Look into the Eyepiece • Use the Coarse Focus

  5. Using High Power • Start focusing using lowest power! • Switch to the next higher objective • Do NOTuse the Coarse Focus Knob • Use ONLY the Fine Focus Knob to bring the slide into focus!

  6. Oil Immersion • Focus on low power (4X objective), focus on 10X, focus on 40X • If using 100x, move to half-way between 40X and 100X (oil immersion) • Add a very small amount of oil (less than 1 drop) • Click 100X objective into place • Do NOT move back to 40X, you’ll get oil on it!

  7. Oil Immersion • Move the 100X lens to front. • CLEAN lens with lens paper. • CLEAN lens only w/ lens cleaner solution & lens paper! Kimwipes are not lens paper • CLEAN slide with Kimwipe or papertowel • NEVER use coarse focus with this lens!!

  8. Oil Immersion • Oil has a similar refractive index as glass • Keeps light rays from bending going through sample • Increases resolution

  9. Lab 2 • You will be working thru the lab & recording your observations. • Parts & use of a microscope (I will only hold you for knowing the 13 parts listed in this powerpoint not the 22 in the manual). • Examining a slid of an “e” • Studying the Depth of focus

  10. Measuring Field of View

  11. Measuring Field of View

  12. Estimating Organism Size • How would you determine the actual size of this organism? • Be quick and dirty, it is longer than wide (always use the long side)

  13. Estimating Organism Size • How would you determine the actual size of this organism?

  14. Making a wet-mount slide Get these materials: • A clean slide • A coverslip • A sample • 1-2 drops of water

  15. Add sample and 1-2 drops of water

  16. Lower a coverslip onto the slide starting at a 45 angle By adding the coverslip at a 45 degree angle any bubbles should move up the coverslip and not become trapped underneath it. e

  17. Completed wet-mount slide should have no bubbles e

  18. Observing Cells • Staining Human Cheek Cells • Add stain before adding coverslip or • Stain cell by adding stain and “pulling” under cover slip with a paper towel • Disposal: Put slide and cover slip in bleach, treat as a biohazard. • Other slides either wash & put away or add to the bleach container. Coverslips are put in the broken glass container.

  19. OBSERVATIONS • DRAW WHAT YOU ACTUALLY SEE, not what you think it looks like. • Make it actual size

  20. Warnings! • CLEAN off oil immersion lens AND the 40x objective when you’re finished w/ lens paper. • Put away the microscope properly as listed on the cabinet. Clean up & return all items, wash glassware. • Discard trash appropriately (if contaminated in bleach, if glass with the broken glass, otherwise in the trash). • Avoid long black “organisms” that move when you blink (your eyelash). Avoid slow-moving circular “cells” with thick black borders (RBC in your eye). • Do not force the microscope & only use coarse focus on low power!

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