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Topic Outline-Photosynthesis

Topic Outline-Photosynthesis. Topic Outline-Photosynthesis (continued). Figure 10-01. Figure 10.1 Photoautotrophs. A reaction with a positive delta G. Is exergonic can occur if an enzyme is present that changes its delta G to a negative value Is spontaneous

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Topic Outline-Photosynthesis

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  1. Topic Outline-Photosynthesis

  2. Topic Outline-Photosynthesis (continued)

  3. Figure 10-01

  4. Figure 10.1 Photoautotrophs

  5. A reaction with a positive delta G • Is exergonic • can occur if an enzyme is present that changes its delta G to a negative value • Is spontaneous • Proceeds from a state of lower free energy to higher free energy • More than one of the above • All of the above

  6. If a reaction is slowed down by an inhibitor and speeds up as more substrate is added; the inhibition is most likely: • Irreversible • Competitive • Noncompetitive • Allosteric • More than one of the above

  7. Substrate level phosphorylation occurs in: • Glycolysis • Citric acid cycle • ETS • More than one of the above • All of the above

  8. This is required to make ATP by oxidative phosphorylation: • An ATPase channel protein • A mitochondrial inner membrane impermeable to H+ ions • Reduced coenzymes • Oxygen • More than one of the above • All of the above

  9. It is possible for pyruvate to be converted into this molecule during cellular metabolism • Acetyl CO-A • Lactic acid • Fats • all of the above • More than one of the above

  10. This is allows the ETS to produced ATP by oxidative phosphorylation: • The ETS carriers are asymetrically distributed within the mitochondrial inner membrane • There are two types of ETS carriers; hydrogen only and electron only • Water spontaneously dissociates in the matrix of the mitochondria • More than one of the above • All of the above

  11. LE 10-4 12 H2O 6 CO2 Reactants: 6 O2 6 H2O C6H12O6 Products:

  12. A summary of Photosynthesis

  13. H2O LE 10-5_1 Light LIGHT REACTIONS Chloroplast

  14. H2O LE 10-5_2 Light LIGHT REACTIONS ATP NADPH Chloroplast O2

  15. H2O CO2 LE 10-5_3 Light NADP+ ADP + P i CALVIN CYCLE LIGHT REACTIONS ATP NADPH Chloroplast [CH2O] (sugar) O2

  16. Leaf cross section Vein Mesophyll Stomata O2 CO2 LE 10-3 Mesophyll cell Chloroplast 5 µm Outer membrane Thylakoid Intermembrane space Thylakoid space Stroma Granum Innermembrane 1 µm

  17. In photosynthesis, which of the following becomes reduced? • Carbon dioxide • Water • NADP • More than one of the above • All of the above

  18. In photosynthesis, what is the source of the electrons that reduce CO2 into glucose? • Oxygen • NADP • ATP • water

  19. Which of the following is not part of the light dependent reactions of photosynthesis? • Splitting of water • Reduction (fixation) of C02 • Reduction of NADP • Formation of ATP • Photophosphorylation

  20. Which of the following occurs in the stroma? • Splitting of water • Reduction (fixation) of C02 • Reduction of NADP • Formation of ATP • Photophosphorylation

  21. As a plant grows, it increases in mass. Where does that increased mass come from? • Water • The soil • Carbon dioxide • sunlight

  22. Figure 10.9 Location and structure of chlorophyll molecules in plants

  23. 1 m (109 nm) 10–3 nm 103 nm 106 nm 10–5 nm 103 m 1 nm Gamma rays Micro- waves Radio waves X-rays Infrared UV LE 10-6 Visible light 650 750 nm 500 550 600 700 450 380 Shorter wavelength Longer wavelength Higher energy Lower energy

  24. Light Reflected light Chloroplast LE 10-7 Absorbed light Granum Transmitted light

  25. Refracting prism White light Photoelectric tube Chlorophyll solution Galvanometer LE 10-8a 0 100 The high transmittance (low absorption) reading indicates that chlorophyll absorbs very little green light. Green light Slit moves to pass light of selected wavelength

  26. Chlorophyll a Chlorophyll b LE 10-9a Carotenoids Absorption of light by chloroplast pigments 400 700 500 600 Wavelength of light (nm) Absorption spectra

  27. in chlorophyll a CH3 in chlorophyll b CHO Porphyrin ring: light-absorbing “head” of molecule; note magnesium atom at center LE 10-10 Hydrocarbon tail: interacts with hydrophobic regions of proteins inside thylakoid membranes of chloroplasts; H atoms not shown

  28. Figure 10.8 Evidence that chloroplast pigments participate in photosynthesis: absorption and action spectra for photosynthesis in an alga

  29. A summary of Photosynthesis

  30. Excited state e– Heat LE 10-11a Energy of electron Photon (fluorescence) Photon Ground state Chlorophyll molecule Excitation of isolated chlorophyll molecule

  31. Excited state e– LE 10-11 Heat Energy of electron Photon (fluorescence) Photon Ground state Chlorophyll molecule Fluorescence Excitation of isolated chlorophyll molecule

  32. Which of the following is true about a spontaneous reaction? • It’s delta G is positive • The free energy of the products is less than that of the reactants • It requires an input of energy • It never requires an enzyme

  33. Of the 36 ATP/glucose produced by aerobic cellular respiration, 32 of them are produced: • By glycolysis • By the citric acid cycle • During the electron transport system • By substrate-level phosphorylation

  34. Chlorophyll: • Is a pigment • Consists of a porphyrin ring and a long hydrocarbon “tail” • Is associated with the thylakoid membranes • More than one of the above • All of the above

  35. Fluroescence: • Refers to light that is reflected by pigments • Refers to light that is transmitted through a leaf • Requires membranes to occur • Is light that is emitted when an excited electron returns to ground state • 1 and 3

  36. Thylakoid Photosystem STROMA Photon Light-harvesting complexes Reaction center Primary electron acceptor LE 10-12 e– Thylakoid membrane Special chlorophyll a molecules Pigment molecules Transfer of energy THYLAKOID SPACE (INTERIOR OF THYLAKOID)

  37. e– ATP e– e– LE 10-14 NADPH e– e– e– Mill makes ATP Photon e– Photon Photosystem II Photosystem I

  38. H2O CO2 Light NADP+ ADP CALVIN CYCLE LIGHT REACTIONS ATP NADPH O2 [CH2O] (sugar) LE 10-13_1 Primary acceptor e– Energy of electrons Light P680 Photosystem II (PS II)

  39. H2O CO2 Light NADP+ ADP CALVIN CYCLE LIGHT REACTIONS ATP NADPH O2 [CH2O] (sugar) LE 10-13_2 Primary acceptor e– H2O 2 H+ + O2 1/2 e– e– Energy of electrons Light P680 Photosystem II (PS II)

  40. H2O CO2 Light NADP+ ADP CALVIN CYCLE LIGHT REACTIONS ATP NADPH O2 [CH2O] (sugar) LE 10-13_3 Primary acceptor Electron transport chain Pq e– H2O Cytochrome complex 2 H+ + O2 1/2 Pc e– e– Energy of electrons Light P680 ATP Photosystem II (PS II)

  41. H2O CO2 Light NADP+ ADP CALVIN CYCLE LIGHT REACTIONS ATP NADPH O2 [CH2O] (sugar) LE 10-13_4 Primary acceptor Primary acceptor Electron transport chain e– Pq e– H2O Cytochrome complex 2 H+ + O2 1/2 Pc e– P700 e– Energy of electrons Light P680 Light ATP Photosystem I (PS I) Photosystem II (PS II)

  42. H2O CO2 Light NADP+ ADP CALVIN CYCLE LIGHT REACTIONS ATP NADPH Electron Transport chain LE 10-13_5 O2 [CH2O] (sugar) Primary acceptor Primary acceptor Electron transport chain Fd e– Pq e– e– e– NADP+ H2O Cytochrome complex 2 H+ + 2 H+ NADP+ reductase + NADPH O2 1/2 Pc e– + H+ P700 Energy of electrons e– Light P680 Light ATP Photosystem I (PS I) Photosystem II (PS II)

  43. H2O CO2 Light NADP+ ADP CALVIN CYCLE LIGHT REACTIONS ATP NADPH O2 [CH2O] (sugar) STROMA (Low H+ concentration) LE 10-17 Cytochrome complex Photosystem I Photosystem II Light NADP+ reductase Light 2 H+ NADP+ + 2H+ Fd NADPH + H+ Pq Pc H2O O2 1/2 THYLAKOID SPACE (High H+ concentration) 2 H+ +2 H+ To Calvin cycle Thylakoid membrane ATP synthase STROMA (Low H+ concentration) ADP + ATP P i H+

  44. Mitochondrion Chloroplast LE 10-16 CHLOROPLAST STRUCTURE MITOCHONDRION STRUCTURE Diffusion H+ Thylakoid space Intermembrane space Electron transport chain Membrane ATP synthase Key Stroma Matrix Higher [H+] Lower [H+] ADP + P i ATP H+

  45. Light reactions Calvin cycle H2O CO2 Light NADP+ ADP LE 10-21 + P i RuBP 3-Phosphoglycerate Photosystem II Electron transport chain Photosystem I ATP G3P Starch (storage) NADPH Amino acids Fatty acids Chloroplast O2 Sucrose (export)

  46. H2O CO2 Input Light (Entering one at a time) 3 NADP+ LE 10-18_1 CO2 ADP CALVIN CYCLE LIGHT REACTIONS ATP Phase 1: Carbon fixation NADPH Rubisco O2 [CH2O] (sugar) 3 P P Short-lived intermediate P 6 3 P P 3-Phosphoglycerate Ribulose bisphosphate (RuBP) 6 ATP 6 ADP CALVIN CYCLE

  47. H2O CO2 Input Light (Entering one at a time) 3 NADP+ CO2 ADP CALVIN CYCLE LIGHT REACTIONS ATP Phase 1: Carbon fixation NADPH Rubisco O2 [CH2O] (sugar) 3 P P Short-lived intermediate LE 10-18_2 P 6 3 P P 3-Phosphoglycerate Ribulose bisphosphate (RuBP) 6 ATP 6 ADP CALVIN CYCLE 6 P P 1,3-Bisphosphoglycerate 6 NADPH 6 NADP+ 6 P i P 6 Glyceraldehyde-3-phosphate (G3P) Phase 2: Reduction 1 P G3P (a sugar) Glucose and other organic compounds Output

  48. H2O CO2 Input Light (Entering one at a time) 3 NADP+ CO2 ADP CALVIN CYCLE LIGHT REACTIONS ATP Phase 1: Carbon fixation NADPH Rubisco O2 [CH2O] (sugar) 3 P P Short-lived intermediate LE 10-18_3 P 6 3 P P 3-Phosphoglycerate Ribulose bisphosphate (RuBP) 6 ATP 6 ADP 3 ADP CALVIN CYCLE 6 P P 3 ATP 1,3-Bisphosphoglycerate 6 NADPH Phase 3: Regeneration of the CO2 acceptor (RuBP) 6 NADP+ 6 P i P 5 G3P P 6 Glyceraldehyde-3-phosphate (G3P) Phase 2: Reduction 1 P G3P (a sugar) Glucose and other organic compounds Output

  49. Citric acid cycle Glycolysis Oxidation phosphorylation ATP ATP ATP Glucose ATP Hexokinase ADP LE 9-9a_1 Glucose-6-phosphate

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