210 likes | 297 Views
Can Color Detect Cancer?. Andrew Rabinovich 12/5/02. Dead or Not?. E – 300% cancerous DEAD. F – 0% cancerous HEALTHY. How To Detect Cancer?. Spectral Information Spetial Information Texture. Spectral Information Analysis. Proper Image Acquisition
E N D
Can Color Detect Cancer? Andrew Rabinovich 12/5/02
Dead or Not? E – 300% cancerous DEAD F – 0% cancerous HEALTHY
How To Detect Cancer? • Spectral Information • Spetial Information Texture
Spectral Information Analysis • Proper Image Acquisition • Pre-processing(image registration) • Color Information Extraction
Image Acquisition RGB vs. Hyperspectral
Image Registration Registering spectral bands with each other is absolutely unavoidable!!! Acquisition system instability & optical aberrations result in spectral stack misalignment
Raw Spectral Data Short Band Pass (Blue) Long Band Pass (Red)
Registration of Multi modal Images • No brightness constancy • Common features at high resolution • Individual features at low resolution • Suppress the individual and extract the common using a high pass filter
Filtered Images Low Band Filtered High Band Filtered
Shi & Tomasi Affine Registration Determine the motion based on an Affine transformation Transformation is found to sub-pixel resolution
Color Models to Extract Spectral Signal • Color Deconvolution • Non-Negative Matrix Factorization • Independent Components Analysis
Discussion • To quantify the separation of spectral signals, each of the dies must be imaged independently and compared with the separated signal • This study was done with RGB, however, Hyperspectral is a MUST