MRC Lab Procedures

1. MRC Lab Procedures

2. Users • Non-User – Have lab personnel operate the SEM • Supervised user – Have training and lab personnel in the lab when individual operates the SEM • Un-Supervised user – Does not need to have lab personnel in the lab during operation and can use during off hours • Super user-Can train others that have had Met 313!

3. Non-User • On-line request • First time use, allow for introduction to lab and sample prep area • Prep samples correctly • Fill out Request Sheet CORRECTLY • Leave samples or later examination or stay during SEM examination • All data will be stored and archived • Clean Sample Stubs

4. Supervised User • Previous SEM training and or class • On-line request for time on the scope • Sample prepped in the appropriate way • Responsible for data collection • All data is saved on the D drives of both computers and will be clean periodically • Clean sample stubs

5. Un-Supervised User • Previous SEM training and or class • On-line request for time on the scope • Requested time can be off hours • Sample prepped in the appropriate way • Responsible for data collection • All data is saved on the D drives of both computers and will be clean periodically • Clean sample stubs

6. Allow time for good data collection • First time samples can be a mini-research project • If prior work has been done, bring to the SEM appointment. • Use optical microscopes to locate areas of interest and mark them • Respect other’s time in the lab • Respect my time in the lab

7. Not all SEM images are sharp and clear. If you just need basic information you can usually obtain that from a blurred image. • Publication images need more care and might require improper sample prep for best results (i.e. over coating)

8. Optical microscopes are available on a first come first serve basis • If you need instruction please let lab personnel know before you use them so as not to interrupt SEM sessions

9. Request Sheet To be filled out only by those that have lab personnel operate the S4700

10. The more information available the better the analysis. Include previous work, operators do not remember each request.

11. Lab Etiquette • No food or drink on the SEM console. • Please do not interrupt a person while operating the scope. • Be aware of how long it will take you to examine your samples. • Clean up your sample prep.