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Enzyme Kinetics II

Enzyme Kinetics II. 10/16/2008. Kinetic data cannot unambiguously establish a reaction mechanism. Although a phenomenological description can be obtained the nature of the reaction intermediates remain indeterminate and other independent measurements are needed .

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Enzyme Kinetics II

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  1. Enzyme Kinetics II 10/16/2008

  2. Kinetic data cannot unambiguously establish a reaction mechanism. Although a phenomenological description can be obtained the nature of the reaction intermediates remain indeterminate and other independent measurements are needed.

  3. Reaction MechanismsA: Sequential Reactions • All substrates must combine with enzyme before reaction can occur

  4. Bisubstrate reactions

  5. Random Bisubstrate Reactions

  6. Ping-Pong Reactions • Group transfer reactions • One or more products released before all substrates added

  7. Inhibition kinetics • There are three types of inhibition kinetics competitive, • mixed and uncompetitive. • Competitive- Where the inhibitor competes with the substrate.

  8. Competitive Inhibition

  9. HIV protease inhibitors

  10. Competitive Inhibition: Lineweaver-Burke Plot

  11. Uncompetitive Inhibition

  12. Uncompetitive Inhibition: Lineweaver-Burke Plot

  13. Mixed inhibition

  14. Mixed inhibition is when the inhibitor binds to the enzyme at a location distinct from the substrate binding site. The binding of the inhibitor will either alter the KM or Vmax or both.

  15. The effect of pH on kinetic parameters

  16. Lecture 17Tuesday 10/21/08Enzyme Kinetics / Exam II Minireview

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