King Lab Yearly Safety Review

1. King Lab Yearly Safety Review by Cammie Haase-Pettingell for March 2005 Disclaimer-This is Jonathan King’s lab in Biology Dept. yearly safety review and not to be substituted for an initial entry orientation. Any questions should be directed to Cammie 3-3545

2. Points be covered • Emergency Action Plan • Eating • Chemical • Radioactive • Biologicals, Blood Borne Pathogens, Infectious Agents • High Voltage CHP GM

3. Responsibility • PI • Ensure that the lab is conducting in a safe manor • This is by the PI or by a representative the safety rep. • Safety Rep. • To conduct incoming safety orientation • Go to safety meetings • Update the lab on new policy • Individual • To follow the above policy CHP GM

4. Basics • No Eating in lab • Can eat in • Tea room • Computer room , • hall and seminar room • No smoking except outside • No mouth pipeting • Story… CHP GM

5. Evacuation Plan CHP GM

6. Fire Close the lab doors • Pull the fire red fire alarm • Located by Stairwells , Elevators • Leave by stairwells • Get to a phone- dial x100 report fire • Don’t hang up till they have hung up • Not responsible to fight a fire CHP GM

7. Unknown fire such as strange smell • If in doubt call x 100 • Pull fire alarm Other scenario • Look around • Call John Fuccilo to ask he know anything • Ex . melted stuff in autoclave CHP GM

8. Evacuation • The equipment halls can’t hear the alarm • As exit ensure people know about the alarm • If can - put experiments to rest- not required you safety is more important • So firefighters • ie do not stumble upon a active electrophoresis. • Just in case not back for a while may not be ruined • Evacuate by stairwell -all are fire stairwells • Meet by the concrete post by bld 66 • even if you think I saw you Check in so we know no one is stuck in the building • Then go off for what ever. • Some one needs to go to the command post say we are all out • by the door toward Main St. - CHP GM

9. Chemicals • MSDS see handout • Chemical safety web training • http://mitehs.traincaster.com/ • Spill kits • 68-340D behind door • 68-322 under sink CHP GM

10. Hoods • Simple Laminar Flow Hood • Ensure the back “slot” in back is clear • have items on lifts so it is not blocked • Simple test for flow • to tape Kimwipe • If malfunctioning- inform fixit Zone -A http://web.mit.edu/facilities/www/ • Rooms air flow and temperature is dependant on the hood. CHP GM

11. Spills • Chemical • Radioactivity • Biological • General • Clear Area • Get on appropriate safety gear • Determine the Scope • Use the appropriated cleaner • Dispose correctly • Look at the Red flip chart by phone CHP GM

12. Chemical Spill • Evacuate area- put sign up • Done appropriate safety - lab coat, eye protection, appropriate gloves • Determine the necessary sorbant, • For acid base or solvent. • Pour around the outside of the spill - mix in Hold breath • I use the dust pan and broom sweep up. • Wipe up residue • Dispose of as waste CHP GM

13. Chemicals and Medical Emergency • Do not block body showers • Eye wash put injured eye down flush for 15 min. • Eyewash or distilled wash with tubing • Needs to be tempered water • Cut and injury- should go to Medical • can be documented and treated properly • Someone collapses • Ensure you are not putting you self in danger • If can rise -call EMT X100 • EMT will be concern that they are not in danger • try to figure what happened CHP GM

14. Remember • Emergency number X100 • If working alone at night • inform some one on the Hall • Or call campus police 3-1212 • Safety is important to everyone. CHP GM

15. Chemical waste- • Waste needs a red tag • Must be closed when not in use. • Most is in electrophoresis room • Silver- silver stain • MEOH- • Gel drying fix. , Transfer solution • Ethidium bromide - • Liquid and tips • Agarose in the dark room • Once full date and should be picked up immediately by law 2 days • If can call don’t date till wedded in link on King links page http://web.mit.edu/environment/environmental/ehs_services/ehs_areas/hazardous_waste/forms/chem_collection.html CHP GM

16. Sharps • Two types • Biological or not chemical waste-gray bin • If it is sharp from a biological lab it can not be just thrown out. • Chemical waste- with red tags • At the Bench • Need to have covers and be covered when not in use. • Labeled CHP GM

17. Radiation ext. 3-2180 • We use 14C and 35S to label rapidly proteins • Disposal designated by 1/2 life • 20-120day 1/2 life -35 S- 88 day 1/2 life • Over 120 day 1/2 life 14C = 5730 yr 1/2 life • Type- dry (paper gloves), hi level aqueous, sharps/epi. • Radioactivity stocks • is stored in the locked refrig and freezer 68-346D • Most experiment are preformed in the 68-346D. • Geiger counters in each side of the lab. • Pancake probes more sensitive • Ensure the battery is off when done. CHP GM

18. Radioactive spill • Clear the area- block off • Ensure they you have lab coat, safety glasses, double gloves, boots? • Monitor the area with Geiger counter • Mark the area- where there are counts • One does not inactivate the radioactive just clean off the solution. • Good agents -Bleach, Radiact wash, Am4OH • Clean till no signal • RPO will do wipe test • If still signal cover till decayed. CHP GM

19. Biological • BL1- E. Coli • low BL2 - Salmonella typhurminium LT2 • Clean up • All bacteria needs to be in activated by before disposal or going to the kitchen • Autoclaving • Chemical, 10% bleach, 1% Westcodyne, Lysol • Waste • Plates go into the bucket autoclaved in big autoclave after kitchen hours. CHP GM

20. Biological spills • Block the area • Let aerosol settle • Safety gear • Clean up by pour/spray disinfectant on glassware • with dusty pan • Put paper towel on it and pour/spray disinfectant on it • Dispose of in the waste. • Look for the new biological spill kits CHP GM

21. Decontaminating the Bench • 70% ethanol is a better agent to decontaminate the bench than 100% ethanol • 70% is better at denaturing the outer membrane and subsequently lysing the cell • 100 % will only causes effects the on the outer membrane but not lysis. CHP GM

22. High Voltage • Any voltage should be treated as if it is dangerous. • AC current is the most dangerous. • Running a PAGE • Start up a gel by connecting the black lead first as a ground and then the red lead. • It should be noted that high power leads should be used when running a sequencing gel with high voltage. • Turn off a power supply by turning down the voltage source, then turn off the power. • After turning off power, disconnect the red (positive) lead first. • If the hazard light is flashing on the power supply, turn it off and determine what the problem is and fix it, or have it fixed. • If someone is unconscious, call x100 which is the emergency phone number at MIT. CHP GM

23. Chemical Hygiene Plan • OSHA right to know • MSDS know how to read • SOP- Standard Operating Procedures • a written procedure for use of every chemical CHP GM

25. Safety is everyone responsibility • Everyone will be responsible for the level 1 check list which I email the pdf • keep check off for a month • Cammie and Shannon are the safety Reps to do an over view • ensure all unlabeled liquid containing item have a label can be cryptic CHP GM