Shipping Kit Instructions for Fertility Laboratories of Colorado

1. Shipping Kit Instructions for Fertility Laboratories of Colorado CLIA# 06D0211513 COLA#5147 FDA#3005811913 Phone: 303-781-2036 Fax: 720-895-8298

2. Your shipping kit should include the following supplies One insulated (Styrofoam) container One Sarstedt cool transport container with small outer Styrofoam case. Place the plastic transport container flat in the freezer to freeze. Cold Pack 2 bundles of tubes – 2 red top tubes and 1 green screw top transfer tube 2 plastic pipettes One 6”x 9” brown envelope labeled “Please read instructions carefully” Patient Instruction Sheet 2 HIV consents One prepaid FedEx return airbill

4. Everything that comes in the kit needs to be returned. Do not discard or dispose of materials.

5. Collection of Blood Collect the blood in two plain red top tubes. DO NOT USE SERUM SEPARATOR TUBES. All specimens (Red Top Tubes and Transfer Tubes) must have the following information on them: Patient’s full name (First and Last) Last 5 digits of social security number Date of birth Date and time of collection Allow the blood to clot completely. Approximately 15 minutes. Centrifuge the samples and use the plastic pipette to separate the serum from the red blood cells. Place the serum into the transfer tube and dispose of the red top tube. Please do not send the whole blood back with your kit. We only need the serum portion of the blood.

6. The Difference Between Whole Blood and Serum

7. Do not ship us whole blood. If you do, your sample will be rejected and you will have to recollect.

8. Unlabeled Specimens Will Not be Accepted!

9. This is What The Final Sample Should Look Like

10. The Correct Way to Repack a Shipping Kit Freeze the serum specimen(s) for approximately 24 hours prior to shipping. Samples are to be frozen before being placed in the Sarstedt container. Place the frozen transfer tube(s) containing the serum in the previously frozen Sarstedt transport container. Place the Sarstedt container in the small Styrofoam case. Close the top and secure it with the rubber band. Place the closed Styrofoam case into the large Biohazard bag. Enclose the patient instruction sheet and any consent forms in the outside pocket of the Biohazard bag. Place the Styrofoam case which should be sealed inside the Biohazard bag inside the larger Styrofoam container lying on its side. Place the gel cool pack into the remaining space. Replace Styrofoam container lid and seal cardboard box back up Place the prepaid FedEx air bill to the outside of the container. Send OVERNIGHT DELIVERY to: Fertility Laboratories of Colorado Attn: Clinical Laboratory – Lower Level 10290 Ridgegate Circle Lone Tree, CO 80124-5526

11. The Correct Way to Repack a Shipping Kit

12. Everything That Comes in The Kit Needs to Be Returned

13. Important Information SEND SAMPLES MONDAY – THURSDAY ONLY. DO NOT SEND SAMPLES ON FRIDAY – FREEZE AND HOLD UNTIL MONDAY TO SHIP. DO NOT WRITE DIRECTLY ON THE OUTSIDE OF THE SHIPPING BOX. DO NOT WRITE DIRECTLY ON ANY OF THE STYROFOAM or OTHER PACKAGING MATERIALS. BE SURE TO HAVE A WITNESS SIGN THE HIV CONSENT FORM IF YOU ARE HAVING COMMUNICABLE DISEASE TESTING DONE. DO NOT TAPE CLOSED ANY OF THE STYROFOAM CONTAINERS. EVERYTHING THAT IS SHIPPED TO YOU IN THE KIT MUST BE RETURNED TO US. REPLACEMENT COST OF MISSING ITEMS WILL BE CHARGED TO YOUR ACCOUNT. PLEASE CALL 303-781-2036 IN THE AFTERNOON FOLLOWING SHIPMENT OF YOUR KIT TO CONFIRM RECEIPT.

14. Do Not Write Directly on Any of the Shipping Equipment

15. Examples of INCORRECT Packing

16. If you need help please call the clinical laboratory at 303-781-2036.Samples sent improperly may be rejected. Colorado Center for Reproductive Medicine 303-788-8300 Dr. William Schoolcraft Dr. Eric Surrey Dr. Deborah Minjarez Dr. Robert Gustofson