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Parvovirus B19 Genotype 2 Plasma, Sourced from the US

Parvovirus B19 Genotype 2 Plasma, Sourced from the US. Dr Sally A. Baylis, Division of Virology NIBSC. Identification of Parvovirus Genotype 2 Plasma. Plasma obtained at NIBSC in 2000, as a diluent for validation of HCV RNA assays

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Parvovirus B19 Genotype 2 Plasma, Sourced from the US

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  1. Parvovirus B19 Genotype 2 Plasma, Sourced from the US Dr Sally A. Baylis, Division of Virology NIBSC

  2. Identification of Parvovirus Genotype 2 Plasma • Plasma obtained at NIBSC in 2000, as a diluent for validation of HCV RNA assays • Tested by conventional PCR using 3 sets of primers of B19V (2002) • Retested in error in 2004, by a consensus real-time PCR for parvovirus B19 – unexpectedly high titre • DNA sequence analysis confirmed that presence of gt2 B19V (Accession # EF216869) • 98.29% nt identity vs IM-81 (4904 bp) • 97.83& nt identity vs A6 (4846 bp) • This plasma was sourced in the US; it is not a single unit

  3. Genotype 2 Titre • The plasma has a titre of 6.2 log10 IU/ml • Agreement between consensus in-house assay & Artus RealArtTM Parvo B19 LC kit) • No amplification plot in Roche assay • 330 mls of the plasma are available • Plasma pools associated with this sample has a B19V loads between 7.6-8.5 log10 IU/ml • Products associated with the pools contain a mixture of gt1 & gt2 B19V • No B19V infectivity can be found in the gt2 plasma

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