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Parvovirus Genotype 2 Sample Materials IM-81 and IM-82

Parvovirus Genotype 2 Sample Materials IM-81 and IM-82. Baxter, Vienna Matthias Gessner. IM-81. EU Source Plasma donation Bleeddate Dec. 2000 11.3 log 10 IU/ml Parvo IgM negative Volume available Vienna: 100 ml

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Parvovirus Genotype 2 Sample Materials IM-81 and IM-82

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  1. Parvovirus Genotype 2Sample Materials IM-81 and IM-82 Baxter, Vienna Matthias Gessner

  2. IM-81 • EU Source Plasma donation • Bleeddate Dec. 2000 • 11.3 log10 IU/ml • Parvo IgM negative • Volume available • Vienna: 100 ml • Taiwan NLFD (Yi-Chen Yang) offered to provide additional volume if required

  3. Characterization • Sequence of the IM-81 genome was determined by direct sequencing of amplified DNA fragments • DNA sequence of the coding genome region (nt 415 to 4670 according to the sequence of strain Au) • 99.1% identical to genotype 2 strain LaLi and 97.7% identical to strain A6 (clone 2) • 90.8% similarity to genotype 3 sequences V9 and D91.1 • 88.9 to 90.8% similarity to genotype 1 sequences (strains Au, Wi, HV, Rm, N8, and Mi) • 138-bp insertion between nt 173 and 174 of the B19 Au sequence • Insertion not contained in the genotype 2 LaLi sequence and in the genotype 3 V9 and D91.1 sequences (genotype 2 A6 sequence of this region is not available) Ref. J. Blümel et al., JOURNAL OF VIROLOGY, 2005, p. 14197–14206

  4. Characterization II • No significant differences between genome concentration and infectious titer of the IM-81 sample and genotype 1 samples • Human sera raised against genotype 1 cross-neutralize genotype 2 in most instances completely or partially suggesting that viral capsids of both genotypes have related antigens Ref. J. Blümel et al., JOURNAL OF VIROLOGY, 2005, p. 14197–14206

  5. IM-82 • Same donor as IM-81 • Bleeddate 4 days after IM-81 • 7.4 log10 IU/ml • Volume available • Vienna: 200 ml

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