1 / 9

BMP5748: Molecular Biology of parasites

BMP5748: Molecular Biology of parasites. Structure of the course.

wray
Download Presentation

BMP5748: Molecular Biology of parasites

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. BMP5748: Molecular Biologyof parasites Structureofthecourse

  2. 23.9. General intro: DNA replication (Chapter Genes 10) Classicpaper: Replication ofkinetoplast DNA maxicircles. Hajduk, Klein, Englund, CellVolume 36, Issue 2, p483–492, February 1984 / Identificationof ORC1/CDC6-interactingfactors in Trypanosomabruceirevealscriticalfeaturesoforiginrecognitioncomplexarchitecture. PLoS One. 2012;7(3):e32674. doi: 10.1371/journal.pone.0032674. Epub 2012 Mar 8. 25.9. Introducingmutationsduring replication: DNA secondarystructures are associatedwithrecombination in major Plasmodiumfalciparumvariablesurfaceantigen gene families. NucleicAcids Res. 2014 Feb;42(4):2270-81. doi: 10.1093/nar/gkt1174. Epub 2013 Nov 18. How to findstartpointsof replication in theapicoplast: Multiple replication origins within the inverted repeat region of the Plasmodium falciparumapicoplast genome are differentially activated. Mol BiochemParasitol. 2005 Jan;139(1):99-106. 30.9. What to do withchromosomeends: Telomerases background Intro, Articles: The unusually large Plasmodium telomerase reverse-transcriptase localizes in a discrete compartment associated with the nucleolus. Nucleic Acids Res. 2005 Feb 18;33(3):1111-22. Telomere length affects the frequency and mechanism of antigenic variation in Trypanosomabrucei. PLoS Pathog.2012;8(8): e1002900. doi: 10.1371/journal.ppat.1002900. 2.10. Genomics: Workingwithgenome databases: TriTrype PlasmoDB. Chararcterizinggenetictraitswithoutwholegenomesequencing: Optimized Multilocus Sequence Typing (MLST) Scheme for Trypanosomacruzi. PLoSNeglTrop Dis. 2014 Aug 28;8(8):e3117

  3. 7.10. Transcription/splicing: General intro (Genes 10) Thebasics: Identification of a novel Y branch structure as an intermediate in trypanosome mRNA processing: evidence for trans splicing. Cell. 1986 Nov 21;47(4):517-25 RNA decay: Transcriptome-wide analysis of trypanosome mRNA decay reveals complex degradation kinetics and suggests a role for co-transcriptional degradation in determining mRNA levels. Mol Microbiol. 2014 Aug 22. doi: 10.1111/mmi.12764. 9.10. RNA editing: The classics: Major transcript of the frameshifted cox2 gene from trypanosome mitochondria contains four nucleotides that are not encoded in the DNA. Cell 46, 819-826 (1986) RNA interference analyses suggest a transcript-specific regulatory role for mitochondrial RNA-binding proteins MRP1 and MRP2 in RNA editing and other RNA processing in Trypanosomabrucei. J Biol Chem. 2005 Jan 28;280(4):2429-38. 14.10. Control is essential: variantgene expression in protozoansReview for Trypanosomabrucei:Antigenicvariation in Africantrypanosomes.Mol Biochem Parasitol. 2014 Jul;195(2):123-129. Antigenic variation in Giardialamblia is regulated by RNA interference. Nature. 2008 Dec 11;456(7223):750-4. Strand-specific RNA-Seq reveals widespread and developmentally regulated transcription of natural antisense transcripts in Plasmodium falciparum. BMC Genomics. 2014 Feb 22;15:150.

  4. 16.10. Novel tools for parasite manipulation: Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system. Nat Biotechnol. 2014 Aug;32(8):819-21. Efficient genome engineering of Toxoplasmagondii using CRISPR/Cas9. PLoS One. 2014 Jun 27;9(6):e100450. Inducible knockdown of Plasmodium gene expression using the glmSribozyme. PLoS One. 2013 Aug 30;8(8):e73783. 21.10. final exam Duringthecourse: Small cloningtasks as self training in basicprocedures“homework”

  5. Structureofthepresentations: • You work in teamsoftwo, presentinggroups are randomlychosenateach data* • Introductions (given in redletters) are presentedbyvolunteers (whodon´thave to prepare thepaperseminars) • Thegroupthatpresentedonepaperwillnotbepresentinganotheronthesameday • Imagine youdidthestudy: “Yousellthefish” • Tell us whythestudywasdone – what´sthegoalofthestudy • Gointodetailsofthemethods (watchyourcolleagues are theremany “??“ in their faces?) • Getthe background (especiallynewpapers) ifthemessagecannotbeunderstoodfromthepaperitself • Use thepicsformthepaperbutaddother figures fromother sources ifthishelps to explaintheeffects/results * Unpreparedpresentations/absencediscountsonepointfrommax 10 ofthe final grade

  6. Aimofthestudy: • discoverthemechanismofallelicexclusion in in var gene transcription in P. falciparum • This is donebymeasuring var gene transcriptionevents in subnuclear level

  7. Cy5 Metodos Plasmideos de transfecção Real time PCR FISH RNA-FISH núcleo Microscopia confocal

More Related