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Adopted from Conclusions made primarily by Kenneth Olsen and Barbara Schaal, but also from Dolores Piperno and Luiz Carv

Inferences of Cassava’s domestication relating to progenitor(s), location, and time. With additional information regarding its current uses worldwide, capacities of various systematic techniques, and use of ancient starch grains as a taxonomic tool.

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Adopted from Conclusions made primarily by Kenneth Olsen and Barbara Schaal, but also from Dolores Piperno and Luiz Carv

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  1. Inferences of Cassava’s domesticationrelating to progenitor(s), location, and time. With additional information regarding its current uses worldwide, capacities of various systematic techniques, and use of ancient starch grains as a taxonomic tool. Adopted from Conclusions made primarily by Kenneth Olsen and Barbara Schaal, but also from Dolores Piperno and Luiz Carvalho Andrew Gifford Gardner

  2. Cassava (Manihot esculenta Crantz ssp. esculentatapioca, manioc, mandioca, yucaA primary calorie source for over 500 million people,the major source of carbohydrates in sub-Saharan Africa,and the sixth most important crop worldwide-but-It affords almost no cash value,has low vitamin and protein values,and is susceptible to pathogens, especially in Africa.Largely ignored by breeding programs and scientists despite its importance.

  3. Systematics of Manihota genus of 98 neotropical species, with habits ranging from herbs to small trees, and extreme morphological plasticity.Morphology-based taxonomic systems have proven to be problematic, especially in elucidating the relationship between cassava and the rest of the genus.Resulted in a “compilospecies” hypothesis

  4. Olsen and Schaal’s generic systematicsa traditional molecular phylogenetic approach, sampling species which had been previously implicated as potential progenitors of cassava.Two major findings:South American and Mesoamerican species for two distinct clades.Cassava is clearly a member of the South American clade, with nearly identical sequences to M. esculenta ssp. Flabellifolia. ITS maximum parsimony tree

  5. Three maindomestication questions:1. Is cassava derived from M. esculenta ssp. Flabellifolia?2. Is M. esculenta ssp. Flabellifolia a feral escape from cultivated cassava? 3. Is cassava only partly derived from M. esculenta ssp. Flabellifolia? In other words, is there evidence of hybridization?

  6. Three main domestication questions: Olsen and Schaal used population genetics to answer these questions:1. Is cassava derived from M. e. Flabellifolia?Supported if cassava’s genetic diversity were a subset of M. e. Flabellifolia’s.

  7. Three main domestication questions: Olsen and Schaal used population genetics to answer these questions:2. Is M. e. Flabellifolia a feral escape from cultivated cassava? Supported if M. e. Flabellifolia’s genetic diversity were a subset of cassava’s.

  8. Three main domestication questions: Olsen and Schaal used population genetics to answer these questions:3. Is cassava only partly derived from M. e. Flabellifolia? In other words, is there evidence of hybridization? Supported if cassava’s genetic diversity were a subset of M. e. Flabellifolia’s, but also contained genetic material not present in M. e. Flabellifolia.

  9. They used low-copy nuclear genes and microsatellites for their analyses.Taxa included were the “core of the core” cassava germplasm collection (20 landraces), 157 M. e. Flabellifolia individuals representing much of the range (within Brazil),and 35 M. pruinosaindividuals where the species is sympatric.

  10. ResultsHaplotype gene tree (G3pdh) □ M. e. Flabellifolia ◊ cassava ○ M. pruinosa The other two genes corroborate the patterns seen in this tree.

  11. ResultsMaximum likelihood tree of microsatellite allele frequencies P - M. Pruinosa The vertical bar represents the cluster of wild populations most closely related to cassava

  12. Results Partially shaded - G3pdh haplotype shared with cassava Fully shaded - At least 2 of 3 nuclear genes share haplotypes with cassava Vertical bars - Populations grouped with cassava in the microsatellite analysis

  13. Three main domestication questions:1. Is cassava derived from M. esculenta ssp. Flabellifolia?YES.2. Is M. esculenta ssp. Flabellifolia a feral escape from cultivated cassava? NO.3. Is cassava only partly derived from M. esculenta ssp. Flabellifolia? In other words, is there evidence of hybridization?NO. (but it may occur between M. pruinosa and M. esculenta ssp. Flabellifolia)

  14. Starch grains through history(or, the work of Dolores Piperno)Elucidation of cassava’s history of domestication has been challenging. Root crops preserve extremely poorly in the humid tropics, and the earliest undisputed macrobotanical remnantsof cassava are from 4,000 BP on the arid coast of Peru.

  15. Starch grains through history(or, the work of Dolores Piperno)fortunately, domesticated cassava starch grains differ greatly from other species, including M. e. Flabellifolia common bean cassava sweet potato

  16. Starch grains through history(or, the work of Dolores Piperno)She is working at Aguadulce rock shelter in Panama.Cassava starch on an edge-ground cobble,which should date to nearly 7,000 BP.

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