1 / 1

Controls for RNAi experiments

Controls for RNAi experiments. PRIOR TO FIRST EXPERIMENTS and FOR ALL EXPERIMENTS optimize transfection conditions and control for transfection efficiency. The BLOCK-iT™ Transfection Control Kit and RNAi Basic Control Kit contain reagents to do this. EARLY EXPERIMENTS:

xanthus-adkins
Download Presentation

Controls for RNAi experiments

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Controls for RNAi experiments PRIOR TO FIRST EXPERIMENTS and FOR ALL EXPERIMENTS optimize transfection conditions and control for transfection efficiency. The BLOCK-iT™ Transfection Control Kit and RNAi Basic Control Kit contain reagents to do this. • EARLY EXPERIMENTS: • Unrelated or scrambled siRNA/shRNA control • Positive and Negative control The BLOCK-iT™ RNAi Basic Control Kit can help assess cells health following gene knockdown • INTERMEDIATE EXPERIMENTS: • All controls in “EARLY EXPERIMENTS” • Multiple d-siRNA/siRNA/shRNA to same target gene • Show effect at mRNA level (LUX™primers/QRT-PCR or Northern) • “Stress response” gene monitoring (PKR or 5’OAS pathways) • OPTIONAL: Western analysis The BLOCK-iT™ RNAi Stress Response Kit can help monitor for activation of toxic pathways • FULL SET OF CONTROLS (publication quality): • All controls in “INTERMEDIATE EXPERIMENTS” • Dose-response curve • Rescue of phenotype • OPTIONAL: Global gene expression- arrays “Whither RNAi” Nature Cell Biology (2003) vol 5(6): 489-490

More Related