Cheng et al. Supplemental figures

1. Cheng et al. Supplemental figures Figure S1 Homozygous diploi fis1 Figure S1. WHI2 mutations in the homozygous FIS1 knockout from the YKO collection. DNA sequence chromatograms of homozygous fis1 strain (BY4743) derived by mating the two haploid FIS1-knockout strains (fis1d1and fis1d2) in the YKO collection reveals both mutation d1 (position 457) and d2 (position 206) in the WHI2 ORF. 457 206 Figure S2 Untreated 173 mM acetic acid Low amino acids Figure S2. Reconstitution of WHI2 in the fis1d1 strain rescued both the cell death and overgrowth phenotypes. A low copy plasmid vector (CEN-URA) expressing WHI2 (via its own endogenous promoter) in fis1d1 restores cell survival and reinstates the ability to reduce growth on low amino acid medium similar to wild-type. A representative of 2 similar independent experiments per condition is shown. 5-fold serial dilutions SCDCSH-URA SCDME-URA Figure S3 Untreated 173 mM acetic acid Figure S3. Newly generated FIS1 knockouts (with wild-type WHI2) are not sensitive to cell death. Death sensitivity of 3 different newly generated FIS1-deletion strains (fis1::URA3) were compared to 3 different URA3+ wild-type control strains. A representative of 2 similar independent experiments is shown. 5-fold serial dilutions SCDCSH-URA