Advisor : Cheng-Hsin Chuang Advisee :Wan-Ting Su Department of Mechanical Engineering

1. Advisor : Cheng-Hsin Chuang Advisee :Wan-Ting Su Department of Mechanical Engineering & Institute of Nanotechnology, Southern Taiwan University, Tainan, TAIWAN Date ﹕2012/04/06 Innovate Food Science and Emerging Technologies 12 (2011) 617-622 A microwire sensor for rapid detection of Escherichia coli K-12 in fresh produce Sungkyun Kim ,Lin Lu, Jae-Hyun Chung ,Kyonghoon Lee ,Yong Li ,Soojin Jun PaperSurvey

2. Outline • PaperSurvey • Sensor Design • Results and discussion • Experimental Result • Future work

3. Sensor Design Fig .1. (a) Microwire sensing unit (b) Power supply and measurement equipments

4. Results and Discussion Fig2.Regression curve for enumeration of E.coli K-12cells Fig3.Stepwise produres for detection of E.coli K-12 DEP force and electroosmotic flow Capillary

5. Simulation Result Fig 4. Sequential simulation of electric field strength

6. Experiment Results Fig. 5. FESEM image of E.coli K-12 cells captured on the microwire. (a)Without and (b) with AC field ﹒

7. Experiment Results Fig. 6. Optimization of the working frequency for detection of E.coli K-12 at 106 CFU/ml Fig .7. Fluorescence intensity of E.coli K-12 in PBS buffer when applied with 20VPP and 3MHz

8. Experiment Results Fig. 8. Selective capture of bactrial cells from E.coli K-12 internalized spinach solution: (a) FI values of E.coli K-12 in spinach and buffer solutions at different concentrations (b) FSEM image of the microwire sensor. Many spinach particles are captured on the wire surface

9. Outline • PaperSurvey • Sensor Design • Results and discussion • Experimental Result • Future work

10. Experimental Experimental Method

11. Experimental Results Applied Time ： 0 min Applied Time ： 2 min Flow Rate ： 0.1 ml/hr Al2O3 Concentration：2 wt% BSA Concentration：1 μM Applied Frequency：10 kHz Apply Voltage：10 Vpp Applied Time ： 16 min Applied Time ： 30 min

12. Outline • PaperSurvey • Sensor Design • Results and discussion • Experimental Result • Future work

13. Future work • PaperSurvey • Experiment by Different Parameter • Experimental Sample Using Bladder Cancer Cell • SEM Training

14. Thank you for your attention!!