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PP 1-24

(A). PP 1-24. CP 1-24. SP 1-24. PP 25-48. CP 25-48. SP 25-48. FP 1-24. RP 1-24. DP 1-24. (D). #umc1658 (10minimum / 16 candidates /11 resolved) + indicates unique pool; ? indicates occlusion pp fp sp rp cp dp +? CloneName

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PP 1-24

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  1. (A) PP 1-24 CP 1-24 SP 1-24 PP 25-48 CP 25-48 SP 25-48 FP 1-24 RP 1-24 DP 1-24 (D) #umc1658 (10minimum / 16 candidates /11 resolved) + indicates unique pool; ? indicates occlusion pp fp sp rp cp dp +? CloneName 46 40 32 38 30 24 + b0276H08 46 42 32 40 30 26 ? b0276J08 46 42 20 40 18 14 + b0275J20 38 46 28 36 18 26 ? b0228N04 38 28 28 18 18 8 + b0226L04 47 37 20 36 19 9 ? b0281E20 47 21 20 20 19 41 + b0279E20 47 1 32 48 31 33 ? b0278A08 4 19 27 23 31 46 + b0022C03 11 37 20 48 31 9 + b0065E20 22 1 25 23 47 26 + b0128A01 30 38 9 20 39 47 + b0179F09 24 42 4 18 28 46 + b0143J04 17 19 47 36 16 18 + b0100C23 26 46 35 24 13 33 + b0156N11 26 42 4 20 30 46 ? b0155J04 FP 25-48 RP 25-48 DP 25-48 PP 4 17 22 11 24 26 38 30 46 47 FP 1 19 21 37 38 28 40 42 46 36 RP 4 20 9 25 27 28 32 35 47 SP 18 20 23 24 38 40 36 48 CP 13 16 18 19 39 30 28 31 47 DP 14 18 8 9 24 26 41 33 46 47 (B) Marker p-umc1658 amplified DNA fragments in PP46, FP40, SP32, RP38, CP30, and DP24. PP + FP = 46 + 40 = 86 - 48 = RP38 PP + SP = 46 + 32 = 88 - 48 = CP30 FP + SP = 40 + 32 = 72 - 48 = DP24 48 is subtracted from any equation total >48 to determine the correct pool number since there are only 48 pools per dimension. Lab Convert translated this clone’s address to 0276H08. (C)

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