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Work package 1 (WP1) Topic: Cyanobacterial Dormant Forms in an Aquatic environment

Work package 1 (WP1) Topic: Cyanobacterial Dormant Forms in an Aquatic environment Objective: Establishment of dormant stage / Induction of akinetes in cyanobacteria. # D4 # D8

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Work package 1 (WP1) Topic: Cyanobacterial Dormant Forms in an Aquatic environment

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  1. Work package 1 (WP1) Topic: Cyanobacterial Dormant Forms in an Aquatic environment Objective: Establishment of dormant stage/ Induction of akinetes in cyanobacteria

  2. # D4 # D8 Basic studies to reveal conditions and mechanisms involved in induction of akinetes formation, dormancy and desiccation: Nutrient Depletion (P), Light, Temp, O2 (hypoxia), K Storage of metabolites [carbohydrates, cyanophycin (N)] – enzymes involved, e.g cyanophycine synthase Mature akinetes Respiration and Photosynthetic apparatus (is there pigments production?) O2 evolution/consumption To collect adequate material for further genomic, proteomic and metabolomic studies.

  3. Akinete formation • K > HL > P • 2. Storage metabolites in akinetes • Cyanophycin the storage protein found in cyanobacteria is found in • akinetes of A. ovalisporum and is enriched in aspartic and arginine amino • acids. • 3. Photosynthesis apparatus • Young akinetes perform photosynthetic capabilities as found for vegetative cells grown in akinete-induced media. • Mature akinetes maintain residual photosynthetic activity even though some components of the photosynthetic apparatus appear to remain intact.

  4. # D5 4. Molecular approach Two genomic libraries were cloned into pCC1Fos. The number of clones per culture: 13,056 fosmids 15,360 fosmids A total of 3481 sequencing reads were made from both library fosmids, using primers T7 and M13(-28) Comparison between Anabaena genome and Aphanizomenon contigs led to the selection of nine fosmids that may contain candidate genes loci.

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