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PRESENTATION DO’S AND

DON’TS. PRESENTATION DO’S AND. Muscle contains proteins of many sizes. Protein kDa Function titin 3000 center myosin in sarcomere dystrophin 400 anchoring to plasma membrane filamin 270 cross-link filaments into gel myosin heavy chain 210 slide filaments

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PRESENTATION DO’S AND

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  1. DON’TS PRESENTATION DO’S AND

  2. Muscle contains proteins of many sizes Protein kDaFunction titin 3000 center myosin in sarcomere dystrophin 400 anchoring to plasma membrane filamin 270 cross-link filaments into gel myosin heavy chain 210 slide filaments spectrin 265 attach filaments to plasma membrane nebulin 107 regulate actin assembly a-actinin 100 bundle filaments gelosin 90 fragment filaments fimbrin 68 bundle filaments actin 42 form filaments tropomyosin 35 strengthen filaments myosin light chain 27 slide filaments troponin (T, I, C) 30, 19, 17 mediate regulation of contraction thymosin 5 sequester actin monomers

  3. Phylogenetic Tree Phylogenetic Tree TUNA MACKEREL SALMON TROUT CARP MINNOW SNAPPER PERCH WALLEYE BASS CATFISH COD HAKE POLLOCK SMELT ANCHOVIES HERRINGS SARDINES FLOUNDER SOLE HALIBUT PIKE STURGEON GAR SHARK Agnatha Chondrichthyes Ostheichthyes Amphibia Reptilia Aves Mammalia OYSTER CLAM MUSSEL SCALLOP OCTOPUS SQUID CRAB LOBSTER SHRIMP Mollusk Arthropod Echinoderm Chordate Deuterostome Protostome Metazoa

  4. The stages of mitosis are shown for the onion root tip and the whitefish blastula. The top three slides show the onion root tip. The first is anaphase where the chromosomes are separating. The second is metaphase where the chromosomes line up on metaphase plate. The third is interphase. The next three pictures are of the whitefish blastula. The first is anaphase where the chromosomes are separating. The second is interphase and the third is telophase.

  5. INTERPHASE

  6. PROPHASE

  7. Methods of transformation • Electroporation • Electrical shock makes cell membranes permeable to DNA • Calcium Chloride/Heat Shock • Chemically-competent cells uptake after heat shock

  8. Methods of transformation • Electroporation • Electrical shock makes cell membranes permeable to DNA • Calcium Chloride/Heat Shock • Chemically-competent cells uptake after heat shock

  9. Ca++ O O CH2 O P O Ca++ O H N CH2 O N O OH Why perform each transformation step? Transformation solution incubation = CaCl2 Positive charge of Ca+2 ions shields negative charge of DNA phosphates

  10. Classification Kingdom Phylum Class Order Family Genus Species Traditional classification based upon traits: morphological behavioral Traditional Systematics and Taxonomy

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