Supplementary figure 1

1. PB Ctrl PB Ctrl 4h 8h 24h 17KD H3 AcK9-14 b-actin 43 KD 120 100 80 60 40 20 0 Supplementary figure 1 A. b III Tubulin F actin Hoechst * B. Average neurite length/cell (µm) Ctrl PB C. ** 160 * 140 Average neurite length/cell (µm) 120 100 80 60 ** ** ** 40 20 0 Ctrl TSA dbcAMP Ctrl TSA dbcAMP Actinomycin D

2. Supplementary figure 2 A. Ctrl TSA Hoechst β III Tubulin B. TSA Ctrl Hoechst β III Tubulin

3. Ctrl TSA PDL Myelin 17 KD H3 AcK9-14 43 KD b-actin Supplementary figure 3 A. ** 120 ** 100 ** Average neurite length/cell (µm) 80 60 40 20 0 dbcAMP Ctrl TSA PB B. 120 * * 100 * Average neurite length/cell (µm) 80 60 40 20 0 Actinomycin D Flavopiridol C.

4. GFP P/CAF Hoechst Supplementary figure Supplementary figure 4 A. 120 RNAi CBP RNAi p300 100 RNAi P/CAF 80 RT-PCR- arbitrary units 60 40 20 0 B. GFP-Scr CBP-p300 siRNA GFP-Scr P/CAF shRNA GFP CBP Hoechst

5. GFP P/CAF Hoechst Supplementary figure 4 C. GFP-Scr+TSA 1ng/ml CBP-p300 siRNA+TSA 1ng/ml GFP-Scr+TSA 1ng/ml P/CAF shRNA+TSA 1ng/ml GFP CBP Hoechst 120 Ctrl 100 CBP/p300 siRNA 80 P/CAF shRNA Pixel intentiy.-arbitrary units 60 ** 40 ** ** ** 20 0 vehicle TSA

6. Supplementary figure 4 D. GFP-Scr CBP-p300 siRNA P/CAF shRNA GFP H3 AcK9-14 E. GFP-Scr+TSA 1ng/ml CBP-p300 siRNA+TSA 1ng/ml P/CAF shRNA+TSA 1ng/ml Hoechst

7. Supplementary figure 4 F. 50 40 30 % of transfected neurons with apoptotic features (fragmented or picnotic nuclei) 20 10 0 GFP-scr P/CAF shRNA CBP/p300 siRNA G. 50 40 30 % of transfected neurons with cleavedCapspase 3 staining 20 10 0 GFP-scr P/CAF shRNA CBP/p300 siRNA

8. Ctrl TSA Supplementary figure 5 A. AcH3 K9-14 p300 Hoechst AcH3 K9-14 CBP Hoechst B. C. D. TSA TSA 80 140 70 120 120 60 ** 100 Average neurite length/cell 100 50 Arbitrary Units 80 80 ** 40 Arbitrary Units 60 60 30 40 40 20 ** 20 20 10 0 0 0 Ctrl TSA High Low High Low AcH3K9-14 AcH3K9-14

9. aTub1a +1 SCG-10 +1 Chl1 +1 L1cam +1 CAP-23 +1 Lgals1 +1 -5000 -4000 -3000 -2000 -1000 Supplementary figure 6 A. p53 putative binding site Trans. starting site

10. 4 4 3 3 2 2 1 1 0 0 4 4 3 3 2 2 1 1 0 0 4 4 3 3 2 2 1 1 0 0 Supplementary figure 6 B. Lcam1 a-Tubulin 1a ** * Fold change TSA / vehicle Fold change TSA / vehicle Ctrl 4h 8h 24h Ctrl 4h 8h 24h CAP 23 SCG10 * Fold change TSA / vehicle Fold change TSA / vehicle Ctrl 4h 8h 24h Ctrl 4h 8h 24h Lgals1 Chl1 * * * ** * * Fold change TSA / vehicle Fold change TSA / vehicle Ctrl 4h 8h 24h Ctrl 4h 8h 24h

11. Supplementary figure 7 A. 50 40 30 % of transfected neurons with apoptotic features (fragmented or pyknotic nuclei) 20 10 0 GFP p53 K-R B. 50 40 30 % of transfected neurons with cleavedactive caspase 3 positive cells 20 10 0 GFP p53 K-R