1 / 29

Mitochondrial DNA

Mitochondrial DNA. Debra Baker Department of Biology West Chester University. Journal Article Upon Which this Presentation is Based…. Import of mitochondrial transcription factor A (TFAM) into rat liver mitochondria stimulates transcription of mitochondrial DNA. Authors. Heike L. Garstka, 1

yanni
Download Presentation

Mitochondrial DNA

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Mitochondrial DNA Debra Baker Department of Biology West Chester University

  2. Journal Article Upon Which this Presentation is Based…. Import of mitochondrial transcription factor A (TFAM) into rat liver mitochondria stimulates transcription of mitochondrial DNA

  3. Authors Heike L. Garstka,1 Wolfgang E. Schmitt,1 Jeanette Schultz,1 Bettina Sogl,1 Barbara Silakowski,2 Acisclo Pérez-Martos,3 Julio Montoya,3 Rudolf J. Wiesner

  4. Mitochondrial DNA

  5. Endosymbiosis Theory

  6. Cross-section of Mitochondrion

  7. Matrilineal Inheritance

  8. The human mitochondrial genome is only 16.6kb and among the smallest. • The circular chromosome was sequenced by Sanger's team in 1981

  9. Replication

  10. How Polypeptides Pass Mitochondrial Membranes

  11. Tim and Tom

  12. Technique Used in Study

  13. Immunoblotting aka Western Blot

  14. Background Info • Proteinase K-deactivates nucleases and contaminating proteins and protects DNA and RNA • Triton X-100- surfactant, breaks down membranes. • Carbonyl cyanide m-chlorophenyl hydrazone • (CCCP) –” causes an uncoupling of the proton gradient that is established during the normal activity of electron carriers in the electron transport chain.”

  15. Results

  16. Normal, Hypo, and Hyper-Thyroid

  17. Wt-TFAM • ∆C-25-TFAM

  18. What is the difference between wt-TFAM and ∆C-25-TFAM?

  19. Wt-TFAM is the wild type • ∆C-25-TFAM is, “A truncated version of TFAM (DC-25-TFAM) was produced lacking 25 amino acids.”

  20. Hypothyroid Rats (Levels 1.5-4X higher than Euthryoid)

  21. With ∆C-25-TFAM

  22. Transcription factor A (TFAM) • Essential for in vitro models • Controls the rate of transcription • Does not follow the in vitro pattern when observed in organelle.

  23. How is Transcription Initiated in Organelle without A(TFAM)?

  24. That was not a Rhetorical Question!

  25. The End

More Related