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Barnes August 2007 - Who 16 – 23 Aug1. Jim Postel, marine technician (UW)2. Lydie Herfort, chief scientist (OHSU)3. Mike Malpezzi (UMCES)4. Tiffany Gregg (OSU)5. Bill Howe (OHSU)6. Suzanna Brauer (OHSU) 23 – 30 Aug1. Jim Postel, marine technician (UW)2. Lydie Herfort, chief scientist (OHSU)3. Mike Malpezzi (UMCES)4. Tiffany Gregg (OSU)5. Dan Murphy (OHSU)6. Karen Wegner (OSHU)
Barnes August 2007 - What Tiffany Gregg / Fred Prahl Transport of polycyclic hydrocarbons (PAHs) with suspended particulate matter from the Columbia River to its estuary(River & ETM)Lydie Herfort / Dan Murphy / Bill Howe / Mike Malpezzi / Karen Wegner/ Peter Zuber / Byron Crump/ Holly SimonMicrobial community structure & gene expression, environmental variables(River, ETM, limit salinity intrusion)Suzanna Brauer / Brad TeboCharacterising rates ofMn oxidation using 54-Mn & 14C-bicarbonate uptake(ETM) Samplingwater air pump (70L)sediment Shipeck grab
Plan for Barnes: where River ETM Neap tide Limit salinity intrusion ETM Spring tide
Locations of river end-member Aim: good river-end member 0 / 7 PSU 0 / 20 PSU Beaver Dock 0 PSU St Helen Confluent Willamette & Columbia Columbia Willamette Sampling surface & bottom water in estuary and surface in river
Plan for Barnes: where River ETM Neap tide Limit salinity intrusion ETM Spring tide
Tide in Astoria (17-29 Aug) and ETM sampling days Neap tide Spring tide S M N S M N S N • Aim: • Role of the ETM as a trap for PAHs • Discriminate particle-attached and free-living microbial communities and gene expression • Rate of Mn oxydation S = South Channel M = Mouth Channel N = North Channel = ETM sampling day Sampling surface & bottom water
Plan for Barnes: where River ETM Neap tide Limit salinity intrusion ETM Spring tide
Tide and limit of salinity intrusion sampling days N S • Aim: • Determine diffusion of microbial populations at the limit of salinity intrusion (0-5 PSU) S = South Channel N = North Channel = sampling day Sampling surface water
Samples to collect = 252 (X6 = total 1512 Sterivex) ETM, surface and Bottom water then separated into Bulk and Free-living bacteria S = surface water B = bottom water