CHEMISTRY IN C.S.I.

1. CRIME SCENE INVESTIGATION CHEMISTRY IN C.S.I. Is the blood always red? Martina STANKOVA 1B group 57 Faculty of chemical and food technology Slovak university of technology

2. SUMMARY • Haemoglobin - the unique compound of blood • Techniques of blood’s detection • Luminescene • Crystal reaction • Catalytic color reaction • Instrumental reaction • Resume of detection techniques CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? Martina STANKOVA, 1B group 57, Faculty of chemical and food technology, Slovak university of technology

3. haemoglobin Structure of human haemoglobin. α protein's subunits β protein'ssubunits iron-containing heme groups CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? Martina STANKOVA, 1B group 57, Faculty of chemical and food technology, Slovak university of technology

4. Techniques of blood’s detection CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? Martina STANKOVA, 1B group 57, Faculty of chemical and food technology, Slovak university of technology

5. Chemiluminescence of Luminol + hν 425 nm 1 CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? Martina STANKOVA, 1B group 57, Faculty of chemical and food technology, Slovak university of technology

6. Luminol – emission Spectrum Detection of blood using Luminol on crime scene Luminol emission spectrum : peak at 425 nm Human eye range : from 380 nm to 780 nm CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? Martina STANKOVA, 1B group 57, Faculty of chemical and food technology, Slovak university of technology

7. Lumiscene – Improved Luminol • Same reaction but better wavelenght and eye sensitivity Color corresponding to wavelenght of 525 nm Lumiscene’s emisson spectrum – peak at 525 nm CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? Martina STANKOVA, 1B group 57, Faculty of chemical and food technology, Slovak university of technology

8. Kastle-meyer test - phenolphthalin CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? Martina STANKOVA, 1B group 57, Faculty of chemical and food technology, Slovak university of technology

9. Negative/Positive kastle-meyer test Negative and positive result to presence of blood CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? Martina STANKOVA, 1B group 57, Faculty of chemical and food technology, Slovak university of technology

10. Resume CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? Martina STANKOVA, 1B group 57, Faculty of chemical and food technology, Slovak university of technology

11. THANK YOU FOR YOUR ATTENTION QUESTIONS? CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? Martina STANKOVA, 1B group 57, Faculty of chemical and food technology, Slovak university of technology

12. references • Dr R V Winchester, XII-Biotech-A-Blood Detection-6; • O. Germain, C. Fregeau, and R.M. Fourney, Evaluation of the Effects of Blood Enhancement Chemicals on the Subsequent Short Tandem Repeat DNA Typing Analysis of Blood Fingerprints; • Shannon M. Casey, Hilary M. Stinnett,Gory Dactyloscopy; • http://www.evidentcrimescene.com/cata/chem/chem.html • http://www.lumiscene.com/indexa.html • http://www.sciencebuddies.org/science-fair-projects/project_ideas/BioChem_p037.shtml • http://www.inreferencetomurder.com/html/crime_scene.html CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? Martina STANKOVA, 1B group 57, Faculty of chemical and food technology, Slovak university of technology