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Exploring Connective Tissue: Cartilage, Bone, and Blood Smear

This educational presentation by Jun Zhou, Ph.D. & M.D. from Zhejiang University School of Medicine, covers the microscopic anatomy of cartilage, bone, and blood smear slides, highlighting features such as hyaline cartilage in the trachea, bone structure including Haversian systems and canaliculi, and blood cell types like erythrocytes, neutrophils, eosinophils, basophils, lymphocytes, monocytes, and platelets. The slides showcase the intricate details of each tissue type, providing a comprehensive understanding of their morphology.

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Exploring Connective Tissue: Cartilage, Bone, and Blood Smear

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  1. Lab 3 Connective tissue (2) Cartilage,Bone and Blood smear Jun Zhou(周俊）, Ph.D & M.D School of Medicine,Zhejiang University

  2. Slides • 1.cartilage ( No.3 trachea) • 2.Bone (No.5) • 3.Blood smear (picture drawing)

  3. Hyaline Cartilage C-shape hyaline cartilage Trachea (No.3)

  4. Hyaline Cartilage Trachea(No.3)：H&E ×40

  5. capsule lacuna perichondrium Isogenous groups Trachea：Hyaline Cartilage H&E ×400

  6. Bone • Haversian system(osteon) • Central canal • Circumferential lamella • Interstitial lamella • Bone lacuna • Bone canaliculus Silver ×100

  7. Haversian system(osteon) • Central canal • Bone lacuna • Bone canaliculus Bone：Silver ×400

  8. perforating (Volkmann’s) cannals Bone：Silver ×100

  9. Blood smear: A drop of blood is placed directly on a glass slide and spread over its surface with the edge of another slide. Stained with Wright’s stain.

  10. Erythrocytes: • 7-8um • Biconcave disc • Stain uniformly with eosin • Neutrophil: • proportion - 50-70%; • Most numerous • Multilobed nucleus • Pale pink granules Blood smear：Wright ×400

  11. Eosinophils. • 2-4% • 8-10 microns morphology • bilobed nucleus large, • uniform eosinophil granules. Blood smear：Wright ×400

  12. Basophils. • Proportion 0.5% • morphology - large, irregular basophil granules obscure the nucleus Blood smear：Basophil Giemsa stain ×400

  13. Lymphocyte: • Intensely stained nucleus • Possess a slight indentation • Small amount of cytoplasm • Pale blue Blood smear：Wright ×400

  14. Monocyte: • nucleus ovoid to kidney shaped • blue-gray Cytoplasm Blood smear：Wright ×400

  15. Platelets: • Small speck-like objectives • Aggregated into small groups • From megakaryocytes in myeloid tissue. Blood smear：Wright ×400

  16. Blood smear：Wright ×400

  17. THE END!

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