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Development of a Murine Perfusion System

Development of a Murine Perfusion System. Nicola Asgill Fredrick Hilliard George Kittos. Objectives. Langendorff perfusion system Requirements: Miniature scale Fluid temperature controlled Inter-changeable components Drug introduction into perfusate. Background.

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Development of a Murine Perfusion System

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  1. Development of a Murine Perfusion System Nicola Asgill Fredrick Hilliard George Kittos

  2. Objectives • Langendorff perfusion system • Requirements: • Miniature scale • Fluid temperature controlled • Inter-changeable components • Drug introduction into perfusate

  3. Background • Langendorff Perfusion System • Simulates natural blood flow • Retrograde fluid flow • Attached to aorta • Examine effects of drug on heart

  4. Background (cont.) • Current technology • ADInstruments • Mouse, rat and guinea pig analysis • BP • ECG • HR • Cardiac output • Flow rate

  5. Considerations for project • Why will this work? • Controlled temperature, flow rate and pressure • Improvement of heat stability • Multi-drug delivery capability • Disposable components • Why is this important? • Possible testing applications: • Drug delivery • Cardiac cell response

  6. Prototype

  7. Peltier Elements Beakers Teflon Polyethylene Peristaltic Pump Tygon Tubing Drug Infusion Apparatus Temperature Sensor Flow Sensor Valves Components

  8. Peltier Element

  9. Peltier Element Test • Goals • Show functionality – Heating capabilities • Calculate Time Constant • To heat from room temperature to 37 ± 1°C • Determine appropriate input voltage

  10. Peltier Element Test • Protocol • 8mL of water in Teflon Beaker • Place on Peltier element • Turn on element and measure temperature at 5 second intervals for 5 minutes

  11. Peltier Element Test • Input Voltage – 8V • Time Constant (25°C - 37°) = 149 sec

  12. Peltier Element Test • Input Voltage – 12V • Time Constant (25°C - 37°) = 86.34 sec

  13. Peltier Element Test • Input Voltage – 15V • Time Constant (25°C - 37°) = 62 sec

  14. Peltier Element Test • Conclusion: • Input Voltage of 8V – longer time to heat sample, needs to be faster to perfusate at constant temp. • Input Voltage of 15V – faster heating, but found that this voltage will cause the device to fail. • Input Voltage of 12V – faster heating and the device did not malfunction

  15. Tygon Tubing Peltier Element Current Work • Heating Perfusate to 37 ± 1°C • Designing and manufacturing transparent heart chamber • Testing Peristaltic Pump • Need a Flow Rate of about 250mL/min

  16. Future Work • Create a LabView Module for controlling and maintaining constant temperature • Acquiring additional components • Assembling Prototype

  17. Thanks Questions?

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