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The 2 nd International Symposium “VERA JOHANIDES” - BIOTECHNOLOGY IN CROATIA BY 2020 . Aquaculture and fish cell technology Višnja Gaurina Srček , Kristina Radošević and Zlatko Kniewald University of Zagreb Faculty of Food Technology and Biotechnology
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The 2ndInternationalSymposium“VERA JOHANIDES” - BIOTECHNOLOGY IN CROATIA BY 2020 Aquacultureandfishcelltechnology Višnja GaurinaSrček, Kristina Radošević and Zlatko Kniewald Universityof Zagreb FacultyofFoodTechnologyandBiotechnology Laboratory for CellTechnology, ApplicationsandBiotransformations
Aquaculture – cultivation of aquatic animals and aquatic plants for recreational or commercial purposes. Possibilities of utilizing aquatic organisms include: Increasing the world’s food supply Identifying novel compounds for the benefit of human health and medical treatment Monitoringaquaticpollution The 2nd International Symposium "VERA JOHANIDES" BIOTECHNOLOGY IN CROATIA BY 2020
1.Increasingthe world’s food supply • SOME FACTS ABOUT WORLD FISHERIES AND AQUACULTURE • reached 62.7 milliontonnesin 2011 • projected to reach 172 milliontonnesin 2021 • aquaculturecontributed 40.1% to theworld total fishproduction • providing 55 millionjobs • supportslivelihoodsof 10-12% oftheworld’s population • exportsoffishandfisheryproducts for 2011 exceeded 125 billion US$ Source: The State of the World Fisheries and Aquaculture 2012, FAO, www.fao.org The 2nd International Symposium "VERA JOHANIDES" BIOTECHNOLOGY IN CROATIA BY 2020
Applications of biotechnology in aquaculture • control of reproduction • growth enhancement • genetic characterization • control of diseases • metabolic engineering • transgenesis The 2nd International Symposium "VERA JOHANIDES" BIOTECHNOLOGY IN CROATIA BY 2020
Table 1. Geneticallymodifiedspeciesbeingtestedinaquaculture The 2nd International Symposium "VERA JOHANIDES" BIOTECHNOLOGY IN CROATIA BY 2020 Source: 2009 PearsonEducationInc.
AquAdvantage® Salmon • AquAdvantage® Salmon (AAS) include a gene from the Chinook salmon, which provides the fish with the potential to grow to market size in half the time of conventional salmon. • AAS will be grown as sterile, all-female populations in land-based facilities with redundant biological and physical containment. As a result, AquAdvantage® Salmon cannot escape or reproduce in the wild and pose no threat to wild salmon populations. • AAS raised in land-based facilities reduce the environmental impact on coastal areas, eliminate the threat of disease transfer from farms to wild fish and grow more fish with less feed. Additionally, facilities located near major consumer markets reduce the environmental impact associated with air and ocean freight. • AquAdvantage® Salmon is the future of salmon aquaculture. http://www.aquabounty.com/products/aquadvantage-295.aspx The 2nd International Symposium "VERA JOHANIDES" BIOTECHNOLOGY IN CROATIA BY 2020
2. Identifyingnovel compounds for the benefit of human health and medical treatment A wide number of aquaticspeciescontain or are suspected to contain compounds of biomedical interest (antibiotics, antiviral molecules, anticancer compounds and insecticides) Examplesof some medicalcompoundsderivedfromaquaticorganisms The 2nd International Symposium "VERA JOHANIDES" BIOTECHNOLOGY IN CROATIA BY 2020
3. Monitoringaquaticpollution • most currenttoxicologicalresearch on fish is largelybased on in vivo studies • during 2008, 1.1 millionindividualfishwereused for researchandregulatorypurposesinthe EU MemberStates • reductionoffishnumber (-37.8%) compared to 2005 (1.75 millionfish) • use offishcells for theassessmentofchemicalrisksandtheimplementationof REACH regulation (EC 1907/2006) The 2nd International Symposium "VERA JOHANIDES" BIOTECHNOLOGY IN CROATIA BY 2020
Fourpillarsoffishcellline use inenvironmentaltoxicology Bols et al. (2005) Useoffishcellsinthetoxicologyandecotoxicologyoffish. Piscinecelllinesinenvironmentaltoxicology. BiochemistryandMolecularBiologyofFishes. Vol 6, pp 44-83. The 2nd International Symposium "VERA JOHANIDES" BIOTECHNOLOGY IN CROATIA BY 2020
Fishcellsintoxicology • primarycells • establishedfishcellline (> 250 differentcelllines) ascertaining mechanisms by which contaminants exert their toxicity determining the relative toxicity of different chemical contaminants evaluating the toxicity of environmental samples The 2nd International Symposium "VERA JOHANIDES" BIOTECHNOLOGY IN CROATIA BY 2020
ResearchinourLaboratory • CCO cell line obtained from ovaries of ChannelCatfish (Ictaluruspunctatus) 1. Optimizationofgrowth parameters for biomassproduction • glucose, glutamine, lactate, ammoniaandamino-acids • growthin serum-freemedium 2. Cytotoxicitytestingsof environmentalpollutants (EDC’s) • cellviability • apoptosis/necrosis The 2nd International Symposium "VERA JOHANIDES" BIOTECHNOLOGY IN CROATIA BY 2020
Thankyou for yourattention The 2nd International Symposium "VERA JOHANIDES" BIOTECHNOLOGY IN CROATIA BY 2020