Name:_______________

1. Protein Y is only expressed Maternally (0-3hrs) and is an activator. Protein Q is only expressed Zygotically (3hrs+) and is an activator. RBP only allows Protein ARX to be made; ARX is a Repressor. Protein AZ is an Activator with 50% binding site occupancy. Quiz 6: Biol 203 Fall 2013 Name:_______________ Lab:_______________ 24pts Contains stop codon Y binding site Q binding site Min Prom Z R X A RBP Binding sites Q binding site Q. Put marks on graphs 1 and 2 where appropriate. For graph 3 please draw and label 4 separate curves: 1G, 1R, 2G, 2R RNA binding protein (RBP) RBP 1G 1R 2G 2R 1G 1R 2G 2R 100% 1G,2G the same With 1R would be slightly lower Reporter 1 AZ ARX ATG STOP ATG STOP GFP IRES RFP Expression levels 2R would Never Accumulate Reporter 2 AZ ARX ATG STOP ATG STOP 0% 0hrs 3 6 9 12 GFP RFP At 1hr (Q null bkgd) At 4hrs (Y null bkgd) WT animal EC 2pts: Mark on gene AZRX where RBP would act on the eventual RNA produced during transcription.