Team E Presents: Micro Fluidic Paper-Based Assay Device

1. Alan Dorsinville, Reading High School Natalie Gibbs, Reading High School Team E Presents:Micro Fluidic Paper-Based Assay Device

2. Outline • Introduction • The Problem and our solution • Background Information • The purpose of μPAD’s • Materials • Procedure • Science Concepts • Results • Team F(Floral Experiment) • Conclusion/Discussion • Further Research • Acknowledgements

3. The Problem • The cost of health care is an issue in America • Testing requires • Time • Money • Insurance • Technical Experience • Etc All of which is inconvenient • Developing Countries

4. The Solution • Micro fluidic paper-based assay devices (μPAD) • A paper diagnostic test • Paper-based devices are • Inexpensive • Quick • Easy to use • Require a small volume of liquid • Lack the use of advanced equipment • Effective and accurate

5. Background Information Your body has many substances including proteins and glucose Glucose Glucose provides energy for your body an all of your movements

6. Protein Proteins are important for growth, tissue repair, and many other bodily functions

7. Purpose • Our purpose is to show we can quantify diagnostic results using a cheap paper device • Our chips are designed to detect glucose and protein in our substances

8. How does it work? • Intermolecular forces • Capillary action • Allows us to direct small amounts of liquid to testing wells

9. Materials • CleWin • Chromatography paper( Whatman) • Printer • Scales, beakers, pipettes • Various chemicals • Infrared Gun • Hot Plate • Scanner • Adobe Photoshop

10. Procedure Part One: Planning • CleWin is a computer program made to design our chips

11. Part Two: Production • Step 1: Printing • The pattern is outlined with wax when printed • Step 2: Place the chips on a hot plate at 150°C • Allows wax to seep through

12. Part Three: Chemistry • This project requires making both a protein and glucose reagent • A chemical reagent is a substance used in a chemical reaction to detect, measure, examine, or produce other substances

13. Glucose Assay • Buffer (pH=6.0) • 0.2 M NaH2PO4 • 0.2 M Na2HPO4 • 0.3 M Trehalose • 0.6 M KI • 30 units/mL HRP • 120 units/mL GO

14. Glucose Assay Mechanism Glucose + Glucose oxidase Gluconic acid + Hydrogen peroxide (H2O2) H2O2 H2O + ½ O2 I- ½ I2 HR Peroxidase Brown color

15. Protein Assay • Part 1: • 0.25 M Citric acid (pH 1.8 buffer) • 184 μL H2O, 16 μL EtOH • Part 2: • 9 mM TBPB • 10 μL H2O, 190 μL EtOH • Protein Mechanism TBPB + protein = Blue color

16. Part Four: Applying the Reagents • Apply 0.2 μL of reagents using a micropipette. Must wait ten minutes • Part Five: Test One

17. Back to the Drawing Board • We made a new design for our second chip on CleWin, printed them, and reapplied the chemical reagents, etc.

18. Creating a sample • The chips are a urine analysis test so we made an artificial urine sample • We made several concentrations glucose and proteins to test

19. Testing Pt. Two and Scanning • We performed eight tests for each of the eleven different concentrations of glucose and protein • After 30 minutes, the chips were scanned into the computer

21. Calibration Curve

23. Team F: Floral Exploration • Team F’s research is focused on the relationship between pollinators and nectar

24. Conclusion and Discussion • We made adjustments to our second chip to create a more effective test • We were not able to quantify, but still proved the chips had the potential to quantify different detectable substances • We were able to rank the glucose concentration of Team F’s nectar solutions

25. Further Research • The μPAD’s serve the same purpose as other urine tests • Scientists are trying to find ways of detecting more diseases with the paper-based analytical device

26. Acknowledgements We would like to thank: • Dr. Scott Phillips • SeeCos Faculty • Chris Daly • Ms. Jody Markley • Mr. Derek James • Ms. Jean Marie Donnelly • UBMS Staff

27. Any Questions?