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Design and construction of a micro-milled fluidic device as part of a DNA biosensor

Design and construction of a micro-milled fluidic device as part of a DNA biosensor. Rosie Townsend Nick Harris David Wenn David Brennan. Introduction. Function and operation of sensor Features of fluidic header (isolation of samples) Fabrication and assembly of header

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Design and construction of a micro-milled fluidic device as part of a DNA biosensor

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  1. Design and construction of a micro-milled fluidic device as part of a DNA biosensor Rosie Townsend Nick Harris David Wenn David Brennan

  2. Introduction • Function and operation of sensor • Features of fluidic header (isolation of samples) • Fabrication and assembly of header • Fluidic design for priming and isolation of samples.

  3. Binding of genetic material Function and principle of operation of biosensor Immobilisation Hybridisation Surface stresses Cantilever deflection Microelectronics Institute of Barcelona (IMB-CNM, CSIC) Lechuga et al. (2006) Sensors and Actuators B: Chemical

  4. Optical detection Function and principle of operation of biosensor • Cantilever bending detected optically • Laser source • VCSEL (vertical cavity surface emission lasers) Lechuga et al. (2006) Sensors and Actuators B: Chemical

  5. Role of Fluidic Header Fluidic Header Purpose of header • Isolation of samples • Disposable • Easily primed • Good optical path • Incorporate multiple inlets • Rapid fabrication Deliver reagent(s) to chip Deliver sample(s) to chip

  6. Header assembly Fabrication and assembly • Flow channels milled into acrylic (PMMA) • Fabricated using a Datron micro-mill • PMMA thermal bonding • PDMS gasket • Assembled and secured with screws • Forms disposable part of header • Channels down to 100μm x100μm

  7. Header Manifold Manifold assembly Fabrication and assembly • Manifold forms permanent part of instrument • Gauge #19 steel tubing • Header press fits onto manifold • Gasket seals around tubes

  8. Fluid channels Fluidic design Common path over chip Multiple discrete paths over chip

  9. Isolation of samples Fluidic design • Common path – no isolation over chip • Multiple path – separate channels feed each cantilever. • Isolate delivery of 20 different nucleic acids, or • Isolate delivery of 20 different samples • UV adhesive forms barrier between each channel and cantilever pair. Acrylic Adhesive Chip (Gasket sealing underside)

  10. Priming Fluidic design Bubbles trapped in inlet channels Acrylic header Chip PDMS gasket Acrylic base

  11. Priming valve Fluidic design No flow or operational flow rate High priming flow rate

  12. Region of valve which is simulated Priming channel Priming valve Fluidic design No pressure and no flow High pressure and flow rate(~0.1ml/s)

  13. Priming valve Fluidic design

  14. Priming Valve Fluidic design

  15. Conclusions • Design of headers integrated into sensor instrument combining chemical and optical sensing techniques. • Micro-mill fabrication. • Fluidic design to manage multiple samples and deliver to sensor array on single chip. • Improve priming and maintain isolation of samples using a flow actuated PDMS valve

  16. Acknowledgements Southampton University Dave Wenn, Nick Harris, Dave Brennan, Neil Grabham EU Contract IST-2001-37239

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