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Collection, Preservation and Labelling of Molluscs

Collection, Preservation and Labelling of Molluscs. BY: Salvo Sarcia Sinogba Ternida Testa. Collection of Molluscs. 2 Types of Collection Dead Collecting Collection of dead mollusc Live Collecting Collection of live mollusc. Instruments for Collection. Allison Scoop

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Collection, Preservation and Labelling of Molluscs

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  1. Collection, Preservation and Labelling of Molluscs BY: Salvo SarciaSinogbaTernidaTesta

  2. Collection of Molluscs 2 Types of Collection • Dead Collecting • Collection of dead mollusc • Live Collecting • Collection of live mollusc

  3. Instruments for Collection • Allison Scoop • Used to collect molluscs from stream bottoms, specifically Campeloma (a freshwater gastropod). • The scoop is triangular in shape (which serves as the basket in order to catch molluscs)

  4. Ferris Hoe • A tool for pulling or turning over of the logs and woods, and digging through rotten leaves around the stumps.

  5. Davis rake drag • It is used by throwing the device to the water (sea/ freshwater) in order to gather substrates. The device is pulled by the rope attached to it. It is tilted and emptied afterwards in order to collect the desired specimens.

  6. Screens, deepers, nets • Used to collect specimens from shallow water.

  7. Van Eeden Scoop • The scoop is designed for collecting freshwater molluscs.

  8. Shovels • Used to dig up clams. • Also called as clam gun.

  9. Clam tube • Used for drilling sediments • Used for collecting clams

  10. Hammer • Can be used for scraping • Can be used for digging • Can be used for turning over rocks

  11. Bales Hook • A rod with one looped end. • Used for pulling and turning over

  12. Water Pump • Used to collect fine-sized molluscs.

  13. Bags and Collecting Containers • Serves as the storage room of collected mollusc specimens

  14. Light • Forceps/ Tweezers • Loupes • Threads

  15. In collecting molluscs • The habitat shall be considered. • For example terrestrial, freshwater or marine. • Shallow or deep phase of the marine or freshwater ecosystem.

  16. A specific tool may only be used to a specific mollusc species. • You cannot use water pump when collecting large-sized molluscs. • A rake is not advisable when collecting fine-sized molluscs.

  17. Source: • http://books.google.com.ph/books?id=-NbmHx93s8gC&pg=PA10&lpg=PA10&dq=collecting+mollusk&source=bl&ots=DrAsVuhXTH&sig=KrcFFt-_SHC0TypCRctT2VSQ2vs&hl=en&sa=X&ei=4LgCUNSCLNCOmQWHiIzrCQ&ved=0CF0Q6AEwCQ#v=onepage&q=collecting%20mollusk&f=true

  18. Preservation of Molluscs • Three Stages of Mollusc Preservation • Narcotization/ Anaesthization • Killing and Fixation • Permanent Preservation

  19. Narcotization/ Anaesthization • Menthol Magnesium chloride: The animals are kept in clean water in an enamel tray / Petri dish / bowl depending on the size of the sample. Powdered menthol or magnesium chloride is sprinkled over the water and covered with a lid. The sample is left undisturbed for at least 12 hours.

  20. Narcotization/ Anaesthization • Alcohol or Chloral hydrate: 70% Ethyl alcohol or 1 % Chloral hydrate is added drop by drop at frequent intervals to water in which animals are kept and ensuring that the sample is covered with a lid.

  21. Killing/ Fixation • The common chemical used for fixation of animals in the field is 4 to 10% neutral formalin solution. • After fixing and soaking the specimen in water, transfer it to 75% alcohol.

  22. Permanent Preservation • Methods of Preservation • Boiling • 90% Alcohol • Freezing • Microwaving • Using insects or sea anemones • Formaldehyde

  23. Boiling • Cleaned by boiling and removing the animal. • Bodies of gastropods are easily removed when hot.

  24. Microwaving • Wrap each shell with towel. • Put the wrapped shell in a dish and microwave. • Carefully remove the bodies by screwing it out.

  25. Labelling Specimens preserved in ethanol vials should be labelled with locality and taxonomic name labels, placed inside the vial with the specimen. Those labels must be printed with ethanol-proof ink. The labels placed inside of specimen vials may be of various sizes, depending upon the size of the vial. The label must fit within the vial. We recommend using pinned specimen label size and format. Basically, use the same types of labels for both pinned specimens, and specimens preserved in ethanol. The only significant difference is that the ink must be ethanol-proof, and the label paper must be alcohol/chemical resistant (e.g.: Forbon, Resistal, 100% rag content, Tyvek).

  26. Labelling • Specimens are labelled the same way as the other organisms. Same rules are applied.

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