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URIMEM, will facilitate biomarker discovery and change the face of medicine. 高友鹤 YOUHE GAO Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences. Advantages of Urine Proteome. Non-invasive Simpler than plasma Reflects changes of plasma Close to kidney and prostate.
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URIMEM, will facilitate biomarker discovery and change the face of medicine 高友鹤 YOUHE GAO Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences
Advantages of Urine Proteome • Non-invasive • Simpler than plasma • Reflects changes of plasma • Close to kidney and prostate
Disadvantages • Big variation • Need more cases to validate
Urine: may even better than blood for biomarker study • Urine • Suppose to be waste • Changes were maintained if not amplified • Blood • Suppose to be homeostatic • Changes were minimized
Problems • More sample • Higher throughput MS • More analyzing power
Problems to save samples • Very diluted • Protein degradation
Possible ways to save • Precipitate: organic solvent consuming • Freeze dry: energy and labor consuming • Extraction: cost • Not suitable for large numbers of samples
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Urimem Urinary proteins dried on a filter and stored in vacuum
Changed the face of medicine • “Hard as it is to believe today, a single concept developed by Dr. Henry Plummer (1874 – 1936) at the beginning of the 20th century changed the face of medicine.” http://www.mayoclinic.org/tradition-heritage/medical-records.html
Changed the face of medicine • The concept was a centralized medical record, stored in a single repository, and capable of traveling with the patient. (1907) • Now • paperless • filmless • chartless http://www.mayoclinic.org/tradition-heritage/medical-records.html
Biobanking: foundation of personalized medicine • Blood • Urine • Skin cells • Organ • Tissue • other things taken from a body Hewitt RE. CurrOpinOncol. 2011 Jan;23(1):112-9. doi: 10.1097/CCO.0b013e32834161b8.
Do we have enough? Why not? • Invasiveness • Storage expense • Limited biological information (hair, nail)
URIMEM is the answer • Invasiveness: You can’t hold your urine for a few hours. • Storage expense: Proteins dried on a membrane can be stored in vacuum even at room temperature for quite a while. • Limited biological information: much more complicated information from the whole body especially from the secretory system
Can we decode urine proteome? • Not yet • But having large number of samples will help
Acknowledgement • Lulu Jia • Xuejiao Liu • Liu Liu • YouheGao高友鹤 • gaoyouhe@pumc.edu.cn • 13910861559 • Department of Nephrology, PUMC Hospital: Mingxi Li • Funding from 973 Project