1 / 1

Proteomics of Neuroregeneration: Discovery of a Biomarker of Brain Repair

Proteomics of Neuroregeneration: Discovery of a Biomarker of Brain Repair.

season
Download Presentation

Proteomics of Neuroregeneration: Discovery of a Biomarker of Brain Repair

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Proteomics of Neuroregeneration:Discovery of a Biomarker of Brain Repair Quantitative proteomics of mouse brain during post-traumatic regeneration2-D gel analysis of proteins in regenerating brain. The enlarged boxes in the 2-D gels show three spot intensities that were significantly increased in the injured hippocampus. High-resolution proteomic data showed strong upregulation of the protein 14-3-3 during healing.We conclude that the isotope-based quantitative proteomic method is a highly useful approach to assess protein expression levels in defined parts of the brain after specific brain injury. AcknowledgementsNSF DMR 0084173, Knut and Alice Wallenberg Foundation (C.L. Nilsson, P.I.) 14-3-3 quantification by use of an isotopic approach. Average peptide ratios were compared between contralateral and injured hippocampus of wildtype (WT) and transgenic(GV)mice. A 2D gel study of mouse hippocampus (a brain region): Identity of the three increased protein isoforms: 14-3-3 Sihlbom, C.; Wilhelmsson, U.; Li, L.; Nilsson C.L. and Pekny, M. « 14-3-3 Expression in Denervated Hippocampus after Entorhinal Cortex Lesion Assessed by Culture-Derived Isotope Tags in Quantitative Proteomics» J. Proteome Res. (2007), 6, 3491-3500.

More Related