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Jenny’s Ix Findings. FBE HB 11 , WCC 7.0, Plt 110 HB 10 , WCC 7.3, Plt 90 HB 9 , WCC 7.2, Plt 96 HB 9.5 , WCC 7.2, Plt 115 UECs Na 140, K 4.0, Ur 7.0 , Cr 110 LFTs Mildly elevated ALT and bilirubin , otherwise normal
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Jenny’s Ix Findings • FBE • HB 11, WCC 7.0, Plt 110 • HB 10, WCC 7.3, Plt 90 • HB 9, WCC 7.2, Plt 96 • HB 9.5, WCC 7.2, Plt 115 • UECs • Na 140, K 4.0, Ur 7.0, Cr 110 • LFTs • Mildly elevated ALT and bilirubin,otherwise normal • Atypical pneumonia - Legionella, Chlamydia species, Mycoplasma pneumoniae, Pneumocystis jiroveci serology - pending • CXR - clear • Malarial Thick and Thin Film • Negative • Positive for plasmodium falciparum, parasite count 0.2% • parasite count 0.1% • parasite count 0% • Hep A serology - Total Ab positive, IgM pending • Hep B serology - Surface Antibody positive, surface pending • Arbovirus (dengue) serology - pending • HIV serology - negative • Pregnancy Test - pending
Malarial Thick and Thin Blood Films • 3 thick and thin smears 12-24hrs apart should be obtained • Highest yield of peripheral parasites occurs during or soon after a fever spike; however smears should not be delayed to await a fever spike. • Thin Films - qualitative (speciation) • Drop of blood on specimen slide • Clean spreader slide held at an angle of 45 degrees over droplet of blood • Spread the blood along the specimen slide evenly and thinly • Wait until blood sample is dry before you fix the sample using methanol, then stain. • Thick Films - quantitative (parasite count) • Drop of blood on specimen slide • Use another clean slide to spread the droplet of blood in a circle approx. 1-2cm in diameter • Wait until blood sample is dry before staining (should not be fixed)
Slides • Plasmodium falciparum • P vivax • P ovale • P malariae • Normal
References • http://emedicine.medscape.com/article/221134-diagnosis - malaria • http://emedicine.medscape.com/article/177484-diagnosis - hep a • http://emedicine.medscape.com/article/215840-diagnosis - dengue • http://emedicine.medscape.com/article/231135-diagnosis - typhoid • Oxford Clinical Handbook 7th edn, pp. 382-385, 394, 414, 421 • PBL book case 14 p 41-43 • http://www.rph.wa.gov.au/malaria/diagnosis.html - pics