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Multimodality small animal imaging: registration of functional EPR images with MRI anatomy. Chad R. Haney, Adrian Parasca, Charles A. Pelizzari, Greg S. Karczmar*, Howard J. Halpern Department of Radiation and Cellular Oncology and *Department of Radiology The University of Chicago.
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Multimodality small animal imaging: registration of functional EPR images with MRI anatomy Chad R. Haney, Adrian Parasca, Charles A. Pelizzari, Greg S. Karczmar*, Howard J. Halpern Department of Radiation and Cellular Oncology and *Department of Radiology The University of Chicago Supported by grants DAMD17-02-1-0034 (DoD) and P41EB002034(NIBIB)
In Vivo EPR Imaging – Topic of NIBIB Research Resource(PI: Howard Halpern, MD, PhD) • Long term goal - develop EPR imaging techniques which provide functional information that can be of use in designing, delivering, and assessing cancer therapy.
Biological imaging to enhance targeting of radiation therapy: oxygen imaging • Intensity modulated radiation therapy allows sophisticated control over spatial distribution of radiation dose • Areas of hypoxia could be given extra dose if we could identify them
Why EPR Imaging? • Spectroscopic Imaging: Specific quantitative sensitivity to Oxygen, Temperature, Viscosity, pH, Thiol • No water background obscures spectrum of interest (vs MRI) • ~600 times stronger coupling to magnetic field, environment (vs MRI) • Deep sensitivity at lower frequency (vs optical) • Noninvasive (vs probes)
EPR in vivo oximetry techniques • Localized spectroscopy with implanted particulate probes (Dartmouth) • Spectroscopic imaging with stepped fixed gradients, water soluble probes • CW (Chicago, OSU, Aberdeen, L’Aquila) • pulsed (NCI, Chicago) • OMRI (NCI, Aberdeen) • dynamic nuclear polarization using EPR spin probes
Fix RF frequency, sweep field or fix field, sweep frequency: EPR is analogous to NMR: Zeeman splitting of electron spin energy states in magnetic field
EPRI is not identical to MRI: • Relaxation times ~10-6 as long • pulsed gradient techniques not applicable • FID correspondingly short → demanding of pulsed measurement techniques π/2 pulse ~50 ns long, FID lasts few μs • have to introduce spin probe – no endogenous signal • frequency ~660 times higher for given field (or, field 660 times lower for given frequency)
RF penetration favors lower frequency proton Larmor frequency = 4258 Hz/gauss 42.6 MHz at 1 Tesla electron Larmor frequency = 2.80 MHz/gauss 28 GHz at 1 Tesla 250 MHz ~ 6 T MRI, 90 G EPR S/N ~ w0.8 ratio meas/calc N~w1.2 IN LOSSY, CONDUCTIVE TISSUE
Continuous wave spectral spatial imaging: each voxel yields a spectrum whose linewidth increases linearly with local oxygen concentration fixed stepped field gradients, swept magnetic field EPR line broadening for current narrow line spin probes: approximately 0.5 mG/torr O2
Line width pO2 calibration Oxygen dependence of lorentzian line width obtained in a series of homogenous solutions of OX31spin probe
(a) With no gradient, a field sweep integrates over all spatial locations. This is a pure spectral projection. (b) A gradient along the x direction couples the spatial and spectral coordinates (the spectrum is shifted linearly with position). Spectral-spatial projection (c) A field sweep now corresponds to a projection along a direction rotated in the spectral-spatial plane. Larger gradients correspond to larger rotation angles. Pure spatial projection would require infinite gradient.
250 MHz Spectrometer Magnetsvarying diameter homogeneous field regions (90 G) Intermediate 15 cm diam. Large 30 cm diam. Small 8 cm diam.
Mouse Image using OX063 spin probe PC3 human prostate cancer xenograft on nude mouse hind limb
Registration of EPR with MRI for anatomically aided analysis Registration based on - Fiducials - Surfaces - Intensity distribution Note high intensity due to poor clearance of spin probe from tumor, and low oxygen tension in same region
Early fiducial markers filled with dilute spin probe solution. Problem: need to remove during 4D image to avoid artifacts
Immobilization cast, fiducial markers for serial and intermodality registration
Manual refinement of initial registration estimate based on fiducials
PC3 tumor treated with Ad.CMV.null virus (control) Pre treatment: mean pO2 in tumor 44.6 torr, std 35.1, SEM 1.62. tumor volume from MRI: 0.160 mL 4 days post treatment (right): mean pO2 in tumor 28.7 torr, std 29.1, SEM 1.065. tumor volume from MRI: 0.422 mL
PC3 tumor treated with Ad.EGR-TNFα virus + 10 Gy Pre treatment: mean pO2 in tumor 27.3, std 36.1, SEM 1.122 tumor volume from MRI: 0.524 mL 4 days post treatment: mean pO2 in tumor 31.7 torr, std 17.1, SEM 0.472. tumor volume from MRI: 0.417 mL
Conclusions • 4D EPR Images can be obtained with ~1 mm spatial resolution and ~1.5mG (~3 torr pO2) spectral resolution • Preliminary images of increased and decreased regional oxygenation levels following radiation + adeno-EGR-TNF anti-vascular therapy have been seen. • These images may have potential for biologically-based planning and assessment of radiation therapy • Registration of these functional images with anatomic images such as MRI is key to accurate interpretation and to eventual clinical applications
Chicago EPRI Lab: Howard Halpern Martyna Elas Colin Mailer Chad Haney Charles Pelizzari Kazuhiro Ichikawa Gene Barth Ben Williams Kang-Hyun Ahn Adrian Parasca VS Subramanian Chicago MRI Lab: Greg Karczmar Jonathan River Xiaobing Fan Marta Zamora Denver EPR Lab: Gareth Eaton Sandra Eaton Richard Quine George Rinard EGRF-TNF radiation therapy: Ralph Weichselbaum Helena Mauceri Michael Beckett