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Depurination

Depurination. Release of adenine or guanine bases. Deamination. Often converts cytosine to uracil. Pyrimidine Dimers. Linkage of adjacent pyrimidine bases. Summary Of DNA Damage. hydrolysis (including depurinations and deaminations) oxidation methylation.

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Depurination

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  1. Depurination • Release of adenine or guanine bases

  2. Deamination • Often converts cytosine to uracil

  3. Pyrimidine Dimers • Linkage of adjacent pyrimidine bases

  4. Summary Of DNA Damage • hydrolysis (including depurinations and deaminations) • oxidation • methylation

  5. Replication Of Deaminated Cytosine

  6. Replication Of Depurinated Nucleotide

  7. Base Excision Repair • Remove damaged base using DNA glycosylase • Cut phosphodiester backbone • Fill in gap

  8. Nucleotide Excision Repair Multienzyme complex • Recognizes bulky lesion • Cuts phosphodiester backbone

  9. Repair of Double Strand Breaks • NHEJ: Ligation of broken ends with loss of sequence • HR: Undamaged homologous chromosome as template

  10. Diseases Of DNA Repair Disease Defective Process Effect Xeroderma pigmentosum Nucleotide excision repair UV sensitivity, skin cancer BRCA-1, BRCA-2 Repair by homologous recombination Breast and ovarian cancer Hereditary nonpolyposis colon cancer Mismatch repair Colon cancer

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