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Bio 9C: Thursday , 2.17.11 Title: RNA Structure and Function

Bio 9C: Thursday , 2.17.11 Title: RNA Structure and Function. Homework: Read pages 302-306 in the textbook . Take active reading notes, paying attention to important vocabulary terms and to the following purpose questions: How does the genetic code work?

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Bio 9C: Thursday , 2.17.11 Title: RNA Structure and Function

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  1. Bio 9C: Thursday, 2.17.11Title: RNA Structure and Function • Homework: • Read pages 302-306 in the textbook. Take active reading notes, paying attention to important vocabulary terms and to the following purpose questions: • How does the genetic code work? • What are the steps of translation? Do Now: • Today’s Objectives: • Describe the major structural differences between DNA and RNA • Differentiate between structure and function differences between the types of RNA

  2. RNA: Structures & Functions PHA Biology 9 Moretti and Dickson

  3. RNA Structure & Function of Different Types

  4. RNA Structure: Base Pairing • 4 Nitrogen Bases in RNA: • Adenine • Guanine • Uracil • Cytosine • RNA –> DNA Base Pairing: • A ---- U • G ---- C • Objectives for Class: • Describe the major structural differences between DNA and RNA • Differentiate between structure and function differences between the types of RNA

  5. RNA Structure: DNAvs. RNA • Objectives for Class: • Describe the major structural differences between DNA and RNA • Differentiate between structure and function differences between the types of RNA

  6. Types of RNA: Overview • Objectives for Class: • Describe the major structural differences between DNA and RNA • Differentiate between structure and function differences between the types of RNA

  7. Types of RNA: Messenger RNA (mRNA) • Basic Structure: • Single Strand of RNA • Basic Function • Make a copy of DNA instructions to make a protein • Takes those instructions from DNA to Ribosome to make a protein • Every 3 bases is a Codon Remember: Small enough to leave the nucleus. DNA is too big! • Objectives for Class: • Describe the major structural differences between DNA and RNA • Differentiate between structure and function differences between the types of RNA

  8. Types of RNA: Transfer RNA (tRNA) • Basic Structure: • Coiled RNA strand • Amino Acid attached to the top • Anti-codon on the bottom • Basic Function: • Transfer amino acids to the ribosome to make a protein • Anti-codon on tRNA matches/ reads codon on mRNA • Objectives for Class: • Describe the major structural differences between DNA and RNA • Differentiate between structure and function differences between the types of RNA

  9. Types of RNA: Transfer RNA (tRNA) • Objectives for Class: • Describe the major structural differences between DNA and RNA • Differentiate between structure and function differences between the types of RNA

  10. Decoding the Genetic Code

  11. The Genetic Code • The 64 Codons that code for Amino Acids • Objectives for Class: • Use the genetic code to translate a DNA or RNA sequence into an amino acid sequence to make a protein.

  12. Reading The Genetic Code • mRNA codons are 3 bases long and specify a particular amino acid. • To use the wheel, the 1st base (letter) of codon matches the biggest letter and you move out from the middle to read the rest of the codon. • Therefore, the 2nd base in the codon is the second biggest letter (the one in the middle ring) and the 3rd base In the codon is the smallest letter (the one in the outer ring). • The arrows show the direction you should read it.

  13. The Genetic Code • Complete Part I of the “Decoding the Genetic Code!” Activity. (we will review the first 3 examples of question 1 after you have had a chance to complete them) • Objectives for Class: • Use the genetic code to translate a DNA or RNA sequence into an amino acid sequence to make a protein.

  14. Reading The Genetic Code: Examples • Follow along with the examples in your packet! • Example 1: CAG • 1st base = C • 2nd base = A • 3rd base = G • Therefore the • amino acid is… • Glutamine! • Example 2: GUU • Valine

  15. Reading The Genetic Code: Longer Example • Example 3: GCCAGCUAG • Step 1: Break it into 3-letter sections (codons) • GCC AGC UAG • Step 2: Decode each codon • GCC = Alanine • AGC = Serine • UAG = STOP(end of protein sequence) 1 3 2

  16. Bio 9C: Friday, 2.18.11Title: Transcription and Translation with RNA • Homework: • No Homework over the break!  • Do Now:In your notebook: • If you had a DNA sequence of T A C A G C T, what would the matching RNA sequence be? • Hit the space bar to see the answer… A U G U C G A • Today’s Objectives: • Demonstrate your understanding of DNA, the Cell Cycle and Mitosis

  17. MCAS Prep Question The mold Aspergillusflavusgrows on grain. A. flavusproduces a toxin that binds to DNA in the bodies of animals that eat the grain. The binding of the toxin to DNA blocks transcription, so it directly interferes with the ability of an animal cell to do which of the following? • Produce ATP using energy released from glucose and other nutrient • transport glucose across the cell membrane into the cytoplasm • Transfer proteins from the endoplasmic reticulum to Golgi complexes • Send protein-building instructions from the nucleus to the cytoplasm and ribosomes

  18. Homework Review Question • Overview: • What is the hidden message in Part II of the homework assignment? • “Give Neighbor High 5” • Objectives for Class: • Describe the purpose and major steps of transcription • Describe the purpose and major steps of translation

  19. Transcription Detail • Overview: • A DNA gene is copied in the form of mRNA • Occurs in the nucleus • Steps: • RNA Polymerase separates DNA strands • RNA Polymerase moves along one of the DNA strands (template) • RNA Polymerase makes mRNA by attaching RNA nucleotides that match the DNA template • Objectives for Class: • Describe the purpose and explain the major steps of transcription. mRNA

  20. Types of RNA: Messenger RNA (mRNA) DNA (the red & green strands) mRNA (the orange strand) • Objectives for Class: • Describe the major structural differences between DNA and RNA • Differentiate between structure and function differences between the types of RNA

  21. Codons&AntiCodons Polypeptide Amino Acid • Codon • 3 base sequence on mRNA that specifies a single amino acid to be added to the polypeptide (protein) • Anticodon • 3 base sequence on tRNA that are match up with the mRNA codon t RNA t RNA Ribosome Anticodon Codon mRNA • Objectives for Class: • Describe

  22. Translation Detail • Overview: • mRNA copy provides instructions for assembling amino acids into a protein • Occurs at the ribosome • Steps: • mRNA enters the ribosome • tRNA molecules bring amino acids to the ribosome • tRNAanticodons match with mRNA codons to put amino acids in the right order • According to the Genetic Code • Objectives for Class: • Describe the purpose and major steps of transcription • Describe the purpose and major steps of translation

  23. Translation • Objectives for Class: • Describe the purpose and major steps of transcription • Describe the purpose and major steps of translation

  24. Transcription & Translation Transcription Translation • Objectives for Class: • Describe the purpose and major steps of transcription • Describe the purpose and major steps of translation

  25. Transcription and Translation: Video • Transcription and Translation Video: 17_13ProteinSynthesis.mpg • Objectives for Class: • Describe the purpose and major steps of transcription • Describe the purpose and major steps of translation

  26. Bio 9C: Monday, 2.28.11Title: Transcribe and Translate a Gene Computer Lab • Homework: • Pass in the codons and amino acid decoding practice homework • Complete Part II of the Transcription & Translation Computer Lab • Do Now: • Sign into a computer and turn around with your back to the computers to wait for your next instructions. • Today’s Objective: • Use the steps of transcription and translation to turn a section of DNA into a protein.

  27. Transcribe and Translate a Gene Computer Lab Procedure: • Log onto http://gslc.genetics.utah.edu/units/basics/transcribe/ • Scroll down to the middle of the homepage and click on the link entitled “Are you ready to transcribe a DNA sequence and translate it into a protein?” • A DNA sequence should appear on the screen. Transcribe the DNA strand by entering the corresponding RNA bases for the original strand. The new mRNA strand will now appear on the screen. • Locate the “start” codon and click on it with your mouse. The mRNA sequence will now move to the top of the field and a flashing box will appear around the “start” codon. • Using the “Universal Genetic Code” to the right, find the amino acid that corresponds with the three-base mRNA codon. Click on the appropriate amino acid and drag it to the corresponding mRNA codon (signaled by the flashing box). Repeat until the mRNA sequence has been Translated. • Answer the Review and Analysis questions for Part I as you complete the lab. • Answer the Review and Analysis questions for Part II after the computer portion of the lab is completed. Transcription Translation • Objectives for Class: • Use the steps of transcription and translation to turn a section of DNA into a protein.

  28. Remember R-groups?

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