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Gratiana boliviana Releases in Georgia and Alabama. Amy Roda and Stephen Hight USDA APHIS PPQ CPHST USDA ARS CMAVE. Goals. Establish populations tolerant of northern conditions Create field insectaries to serve as source for other northern locations
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Gratiana boliviana Releases in Georgia and Alabama Amy Roda and Stephen Hight USDA APHIS PPQ CPHST USDA ARS CMAVE
Goals • Establish populations tolerant of northern conditions • Create field insectaries to serve as source for other northern locations • Develop a method to evaluate the impact of Gratiana boliviana • Evaluate the impact of Gratiana boliviana
Evaluation -1x1 m patch • Count all plants • Measure the five tallest plants • % composition that TSA • Count fruits • Photo • GPS center
Evaluation -100’ Line 2005 # of branches crossing line 2006 added 1 m swath each side line height of plants % TSA of area # fruits & flowers # larvae & adults
Kenny’s Site, Coffee Co. AL 2005 • 2,650 beetles released • Transect set – 2 lines with 6 1x1 m patches 2006 • Beetles survived winter • 2,350 beetles released 2007 • Beetles survived winter, located on site edges • No beetles released June 05 June 06 June 07
Brook’s Site, Geneva Co. AL 2005 • 2,650 beetles released 2006 • Beetles survived winter despite cultural practices • 2,350 beetles released • Transects set – 6 lines 2007 • Beetles survived winter, located ca. 100 m from release • 1,850 beetles released site edges June 05 June 06
Mapleville Site, Chilton Co. AL 2006 • 3,300 beetles released Aug. • Individual plants measured (height, width, length) 2007 • Beetles survived winter • 3,000 beetles released • Transects set – 4 lines August 06 June 07
Herd Farm-Trail Site, Bainbridge, GA 2005 • 2,600 beetles Decatur Co. • Transect lines – only crossings counted • 1X1 m patches 2006 • Beetles survived winter/cultural practices • 1,000beetles released 2007 • No beetles found • No beetles released June 05 June 05 June 06 June 07 June 06
Herd Farm-Pond Site, Bainbridge, GA 2006 • Site located in Aug • Transects set – 4 lines • 1,000beetles released 2007 • Beetles survived winter/cattle • 5,250 beetles released in surrounding area August 06 June 07
Late November Site Visits • All stages present • Plant die back • Patches green material Coffee Co. AL 12/1/06 Chilton Co. AL 11/30/06 Chilton Co. 12/1/ 06 Geneva Co. GA 11/30/06
Future Efforts • Continue evaluation of sites • Discontinue release of G. boliviana • Determine impact of over-wintering population • Release new agents • Bainbridge site/new sites
Questions/Discussion • When do we consider the beetles to be “established”? • TSA plants appear very ephemeral & affected by environmental conditions (drought, farm practice, cows, etc.). How do we measure impact of the beetles? • How do we measure seasonal population changes when plots no longer have TSA? • Grant: Explore how biological control of a weed that serves as a virus reservoir helps reduce the virus spread to commercial crops.