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Energetic Metabolism. Observing the Effects of Respiration and Photosynthesis. Interconnections: Photosynthesis & Respiration. Measure O 2 use. Measure O 2 production. Chemical Equations. Aerobic Respiration C 6 H 12 O 6 + 6O 2 6CO 2 + 6H 2 O + energy
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Energetic Metabolism Observing the Effects of Respiration and Photosynthesis
Measure O2 use Measure O2 production Chemical Equations • Aerobic RespirationC6H12O6 + 6O2 6CO2 + 6H2O + energy • Photosynthesis6CO2 + 6H2O + light energy C6H12O6 + 6O2
Metabolic Rate • Speed of aerobic respiration in an organism (use of O2) • Measured in Calories • unit of energy • amount of heat required to increase temperature of 1 kg of H2O by 1oC • 1 liter of O2 consumption = 4.825 Calories • Our experiment measures metabolic rate asmL O2/gram/day, which can be converted to calories
Factors Affecting Metabolic Rate • Body Temperature Mealworms are ectothermic animals Hypotheses: Do plants or animals have a higher metabolic rate?How do environmental temperatures influence metabolic rates?
Pipettes for mealworms: 5-mL at both 15 and 30oC mealworms peas cheesecloth soda lime Experimental Set Up Pipettes for peas: 1-mL at 15oC 5-mL at 30oC
Reading the Respirometer Pipettes For 1-mL pipette 0.1 mL between numbers 0.01 mL between smallest lines For 5-mL pipette 1 mL between numbers 0.1 mL between smallest lines 1 mL 0.1 mL
2 3.5 mL30 min x 60 min 1 hour x 24 hours 1 day 1 Calculations • Suppose the total amount of O2 consumed in 30 minutesby 15.6 grams of mealworms was 3.5 mL. • Calculation of mL O2/gram/day 3.5 mL30 min x 60 min 1 hour x 24 hours 1 day x _____1______weight in grams x 1 = 15.6 grams = 10.8 ml O2/gram/day
Graphing: Histogram Plot the class averages for pea and mealworm metabolic rates at both temperatures.
More Calculations (see page 62) 150 x 453.6 =68040 grams 2.1 mLgram day x 4.825 calories 1000 mL x 68040 grams • Suppose the metabolic rate of your peas was 2.1 mL O2/ gram/day • Calculation of the calorie intake of a 150 pound student whose metabolic rate matches the peas 2.1 mLgram day x 4.825 calories 1000 mL x 68040 grams = 689.4 calories/day
Photosynthesis Experiment HypothesisWill the rate of photosynthesis be increased by increased light energy?
Syringe 1-mL pipette Elodea bicarbonatesolution Experimental Set Up For 1-mL pipette 0.1 mL between numbers 0.01 mL between smallest lines
Graphing Photosynthesis Data Plot the oxygen produced for light at 50 cm and 25 cm for each timed reading.
Graphing Photosynthesis Data Use separate lines to connect the sets of points.
CLEAN UP! • Return your mealworms, peas, and elodea to the appropriate containers • Put used soda lime in discard bottle • Clean and dry your respirometers. • Discard cheesecloth into the trash • Make sure the sinks stay clean
Worksheet • Complete pages 59-62 from lab manual • Be sure your instructor checks your completed worksheet before you leave the lab