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Team Stritch: Metal-o-coli Removal of Heavy Metal from Contaminated Water

Team Stritch: Metal-o-coli Removal of Heavy Metal from Contaminated Water. Susmita Acharya and Nighat Kokan (Chemistry/Biology Collaboration) Cardinal Stritch University Milwaukee, Wisconsin. Project Goals/Ideas. Application Based

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Team Stritch: Metal-o-coli Removal of Heavy Metal from Contaminated Water

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  1. Team Stritch: Metal-o-coliRemoval of Heavy Metal from Contaminated Water SusmitaAcharya and Nighat Kokan (Chemistry/Biology Collaboration) Cardinal Stritch University Milwaukee, Wisconsin

  2. Project Goals/Ideas Application Based • Understanding synthetic biology and its real world application Our focus • Environmental problem and issues, specifically water contamination by heavy metals

  3. Project Proposal • Two projects that really interested us after reviewing the 2009 iGEM website were • Team UQ-Australia: Mercury sequestration using multicomponentoperon • Team Virginia: Arsenic sequestration for ground water decontamination • Team Stritch: Removal of heavy metals from contaminated water specifically mercury and arsenic

  4. Implementation Strategy • As independent student research projects in collaboration with biology and chemistry faculty • Students have 4 plus credits for independent research study • One credit • literature review, bioethical issues, and research proposal • Three credits • Working on synthetic biology projects • Writing their research paper • Poster and oral presentation at the Institution level and at regional conferences

  5. Metal Resistance Systems in Bacteria • Resistance genes on plasmids or chromosome • For example in mercury resistance • The system consists of • A metal transporter • A metal binding protein • Metal responsive promoter • Reduction mechanism, Hg (II) Hg (0)

  6. Project Design • Overview Diagram from: iGEM 2009 Team UQ Australia

  7. Project Design Plasmid 1 • Allows Hg (II) transportation into cell • Metallothionien protein aids intracellular accumulation • MerR is a transcriptional regulator • MerAreductase (missing) no efflux of Hg (0) Diagram from: iGEM 2009 Projects Team UQ Australia

  8. Project Design Plasmid 2 • MerR promoter linked to Ag43 • Ag43, a native protein to E.coli (E. coli K12) is removed from genome and reinserted on a plasmid which can be controlled by Hg (II) • When Ecoli cells express Ag 43 they clump • Measure using Spectrophotometer Diagram from: iGEM Team UQ Australia

  9. BioBricks Parts • BBa_I76101 (did not work ) • BBa_I728456 MerRT: Mercury inducible promoter +RBS • BBa_J63010 vector • Ag43 isolated from plasmid pKKJ143 • MerT and MerP from plasmid pSUTp • BBa_K205004 (MerT) • BBa_I728654 (mercury Induced RFP reporter)

  10. Long Term Goals • Expand present project to other heavy metal sequestration • To offer synthetic biology as a 2-3 credit project based interdisciplinary course • Design and construct BioBricks • Characterize DNA-based devices • Contribute to registry

  11. Acknowledgements • Our Thanks to • Malcolm Campbell • Laurie Heyer, Todd Eckdahl, and Jeff Poet • Davidson College

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