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Understanding Cellular Respiration Process in Biology Lab

Cellular respiration is a vital process where cells convert organic molecules into ATP for energy. The stages include Glycolysis, Krebs Cycle, and Electron Transport Chain. Aerobic respiration yields 36 ATP per glucose, while anaerobic respiration results in lactate or alcoholic fermentation. A practical lab experiment using yeast demonstrates these concepts. Stay tuned for the upcoming Blood Film lab session.

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Understanding Cellular Respiration Process in Biology Lab

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  1. KURDISTAN REGIONAL GOVERNMENT -IRAQ MINISTRY OF HIGHER EDUCATION & SCIENTIFIC RESEARCH SALAHADDIN UNIVERSITY -ERBIL Cellular Respiration 8thLab of Cell Biology 1st Semester 2022-2023 Azheen S. Abdulrahman M.Sc. in Histology College of Science-Biology Department azheen.abdulrahman@su.edu.krd ORCID: https://orcid.org/0000-0001-8584-2458 

  2. What is Cellular Respiration? • Cellular respiration is the process that cells use to transfer energy from the organic molecules in food to ATP. •C6H12O6+ 6 O2→ 6 CO2+ 6 H2O + 36 ATP

  3. Stored Energy Energy Released Energy had been used

  4. Cellular Respiration Stage One: Glycolysis •Glycolysis Glucose is broken down to pyruvate during glycolysis, making some ATP.

  5. Cellular Respiration Stage Two: Krebs Cycle take place in the matrix of mitochondria. Krebs cycle is aerobic. products are CO2, ATP, NADH and FADH.

  6. Cellular Respiration Stage three: Electron Transport Chain take place in inner mitochondrial membrane, produces 32 ATP. requires oxygen directly (aerobic respiration) the products are H2O and ATP.

  7. AEROBIC RESPIRATION Glycolysis = 2 ATP’s Krebs Cycle = 2 ATP’s Electron Transport Chain =32 ATP’s TOTAL = 36 ATP’s for every Glucose!  

  8. Anaerobic Respiration Lactate fermentation Glucose → energy + Lactic Acid + Carbon dioxide

  9. Anaerobic Respiration Alcoholic fermentation Glucose → energy + Ethanol + Carbon dioxide

  10. Materials

  11. Procedure • Get four test tubes or flask and label them A, B and C.D • Put water in tube A • Put water and yeast in tube B • Put water, sugar and yeast in tube C • Put water, two fold of sugar and yeast in tube D • Close the opening tubes with balloon • Wait for 1hr • Note the result.

  12. Next Lab • Blood Film

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