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Taxonomy. Definition Binomial Nomenclature Genus Specific epithet Strains. Classification. Heirarchy Tools – Dichotomous Key. 5 Kingdom Classification. Whittaker 4 Eukaryotic Kingdoms 1 Prokaryotic Kingdom, now revised (not detailed enough). Kingdom Protista.
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Taxonomy • Definition • Binomial Nomenclature • Genus • Specific epithet • Strains
Classification • Heirarchy • Tools – Dichotomous Key
5 Kingdom Classification • Whittaker • 4 Eukaryotic Kingdoms • 1 Prokaryotic Kingdom, now revised • (not detailed enough)
Kingdom Protista • Single celled, no cell wall • Chemoheterotrophic • Some may photosynthesize (Algae) • Environmental and pathogens
Kingdom Fungi • Single and multi-celled • Chemoheterotrophs • Chitin in cell wall • Spore formers • Many pathogens and decomposers
Kingdom Plantae • Multicellular • Photoautotrophs • Metabolites may be used medicinally • Cellulose in cell wall
Kingdom Animalia • Multicellular • Chemoheterotrophs • No cell walls • Develop from zygotes • Parasites and vectors
3 Domain Classification • Bacteria • Archaea • Eukarya
Bacteria • Prokaryotic size and cell features • Peptidoglycan in cell wall • 70S ribosomes • Membrane lipids like Euks. • No histones
Archaeans • Prokaryotic size and cell features • No Peptidoglycan in cell wall • 80S ribosomes • Membrane lipid differences • Histone-like proteins • Methanogens, halophiles, thermophiles
Evolution • “Tree” versus “Shrub” suggests a community of ancestral cells • Lateral gene transfer • Mitochondria • Chloroplasts • ? Additional gene sequences
Virus Classification • No Kingdoms • Classification characteristics • Nucleic acid • Morphology • All pathogens
Taxonomic Methods • Numerical Taxonomy • Computerized comparison of many genetic characteristics • Genetic Homology • DNA/RNA sequencing • Protein sequencing • PAGE
Taxonomic Methods • Ribosomal gene sequencing • Immunology • Phage typing
Classification Total metabolic and structural detail Reference for new organisms Bergey’s Manual of Systematic Bacteriology Identification Taxonomic key approach Reference for rapidly determining an unknown (e.g. diagnostic work) Bergey’s Manual of Determinative Bacteriology Bacterial Taxonomy