CHO expression

1. 2020/9/4 Mammalian Cell Expression - Profacgen  (https://www.profacgen.com) Home (/) Services Products APP & TECH Contact Us About Us (https://www.profacgen.com/contact- (https://www.profacgen.com/about- us.htm) us.htm) Mammalian Cell Expression Home (http://www.profacgen.com) / Services / Protein Production (https://www.profacgen.com/protein-production.htm)  Sign In (https://www.profacgen.com/SignIn.aspx) / Register / Protein Expression (https://www.profacgen.com/protein-expression.htm) / Mammalian Cell Expression (https://www.profacgen.com/SignUp.aspx) https://www.profacgen.com/Mammalian-Cell-Expression.htm 1/6

2. 2020/9/4 Mammalian Cell Expression - Profacgen Mammalian Cell Expression Mammalian cell expression systems are the best choice for the production of eukaryotic proteins, especially when correct folding and post-translational modification (glycosylation, phosphorylation, etc.) is required. They produce eukaryotic recombinant proteins in the most natural state, with native tertiary structure, physiochemical characteristics, and bioactivities. They have been successfully applied in the biopharmaceutical production of cytokines, monoclonal antibodies, growth factors and so on. The most widely-used mammalian cell lines are HEK293 and CHO cells. Features Protein production with accurate post-translational modification Bioactive protein production Transient and stable expression Large-scale protein production https://www.profacgen.com/Mammalian-Cell-Expression.htm 2/6

3. 2020/9/4 Mammalian Cell Expression - Profacgen Human Embryonal Kidney 293 (HEK293) cells have been extensively used for the transient expression of recombinant proteins. Their ease of culture and highly efficient transfection rate make them especially suitable for customized protein production for research use. Human Embryonic Kidney 293 (HEK293) cells are a specific cell line originally deerved from human embryonic kidney cells grown in tissue culture. They are very easy to grow and transfect very readily, thus widely used in cell biology research and biotechnology industry. Chinese Hamster Ovary (CHO) cells, which have been successfully used for the manufacture of FDA-approved therapeutic proteins and antibodies, are prominent in stable expression. Stably transfected CHO cell line is the most suitable mammalian host for high-yield production of recombinant proteins. They are adaptable to diverse culture conditions. Protein purification from CHO culture is also relatively easy because of their secretory feature. https://www.profacgen.com/Mammalian-Cell-Expression.htm 3/6

4. 2020/9/4 Mammalian Cell Expression - Profacgen Chinese hamster ovary(CHO) cells are a cell line derived from the ovary of the chinese hamster. They’re often used in biological and medical research. Profacgen provides you with an ultimate solution of mammalian protein production. Our service can be tailored with your specialized requirements. Our expertise guarantees high protein quality, short turnaround time, and the best price in the market! See more details on the principle and protocol of mammalian cell expression system on our website. See more... (/recombinant-protein-expression-in-mammalian- cells.htm) SERVICES  Protein Production (https://www.profacgen.com/protein-production.htm)  Anaerobic Fermentation (https://www.profacgen.com/anaerobic-fermentatio…  * Antimicrobial Peptides Services (https://www.profacgen.com/services/protein… * Cell Line Development (https://www.profacgen.com/services/protein-product…  * Custom Lysate Preparation Service (https://www.profacgen.com/Custom-Lys… * Process and Production (https://www.profacgen.com/services/protein-produ…  * Protein Expression (https://www.profacgen.com/protein-expression.htm)  * https://www.profacgen.com/Mammalian-Cell-Expression.htm 4/6

5. 2020/9/4 Mammalian Cell Expression - Profacgen Corynebacterium glutamicum Expression Systems (https://www.profacgen.com/… * Animal-Free Expression (https://www.profacgen.com/Animal-free-Expression.ht…  * Bacteria Expression (https://www.profacgen.com/bacteria-expression.htm) * Baculovirus-Mammalian cell Protein Expression System (https://www.profacgen.… * Cell-Free Protein Expression (https://www.profacgen.com/Cell-free-protein-expr…  * Fusion Protein Expression (https://www.profacgen.com/fusion-protein-expressio… * Insect Cell Expression (https://www.profacgen.com/Insect-Cell-Expression.htm) * Mammalian Cell Expression (https://www.profacgen.com/Mammalian-Cell-Expr… * NovEgg Expression System (https://www.profacgen.com/NovEgg-Expression-Sys… * Protein co-expression (https://www.profacgen.com/services/protein-production/…  * Trimeric Protein Production Service (https://www.profacgen.com/trimeric-protei… * Yeast Expression (https://www.profacgen.com/Yeast-Expression.htm) * Antibiotic-free expression system (https://www.profacgen.com/services/protein-… * Protein Labeling (https://www.profacgen.com/protein-labeling.htm)  * Protein Mutagenesis (https://www.profacgen.com/Protein-Mutagenesis.htm) * Protein Mutant Library Construction (https://www.profacgen.com/Protein-Mu… *  Bioinformatics Platform Introduction  (https://www.profacgen.com/Bioinformatics-Platform-Introduction.htm)  Protein Analysis (https://www.profacgen.com/protein-analysis.htm)   Drug Development (https://www.profacgen.com/drug-development.htm)  INQUIRY (https://www.profacgen.com/order.htm?i1=Mammalian Cell Expression) CONTACT US  Email: info@profacgen.com  Tel: 1-631-448-8149  Fax: 1-631-938-8127  45-1 Ramsey Road, Shirley, NY 11967, USA https://www.profacgen.com/Mammalian-Cell-Expression.htm 5/6

6. 2020/9/4 Mammalian Cell Expression - Profacgen Copyright ©2010 - 2020 Profacgen. All rights reserved. | Terms and Conditions (https://www.profacgen.com/term-and- conditions.htm) | Privacy Policy (https://www.profacgen.com/privacy-policy.htm) https://www.profacgen.com/Mammalian-Cell-Expression.htm 6/6