Perfused Bioreactor with Matrix-Enabled Capillary Scaffold (MECS)

1. Perfused Bioreactor with Matrix-Enabled Capillary Scaffold (MECS) Team Members: Allyson Fry Bryan Gorman Jonathan Lin William Wong Advisor: Dr. John P. Wikswo A continuation from 2004-2005 (Barnett, Garrett, Harvill, Mayer, McClintock)

2. Objective Design a tissue engineering scaffold and supporting microfluidic control system to guide the development of a network of capillary tubes in vitro.

3. Introduction • Bioreactor • Capillary network • Matrix-Enabled Capillary Scaffold (MECS) • Perfusion

4. Motivation • Simplified model for disease research • Human system • Control at many levels • Variety of uses http://www.kids4research.org/mouse%20in%20hand%20facing%20left.jpg

5. Martins-Green a a a a

6. Bioreactor Design Supply Network Support Filter Collagen MECS Collagen Support Filter Supply Network

7. Overall Superstructure

8. Fractal pattern of vasculature

9. Matrix-Enabled Capillary Scaffold

10. Use two-layer photolithography to create mold of ‘egg crate’ design Use soft lithography to create a PDMS stamp Use stamp to deposit a sacrificial layer between two layers of collagen Fabrication Strategy

11. Overall fluidics

12. Pumps and LabVIEW

13. Main Control Program Histories of graph values Graphs pH ranges PID coefficients pH sensor settings Pump settings

14. Main control program

15. Project Goals • Model a complete microvascular network • Support cellular processes • Allow evaluation of molecular mechanisms • Provide environment for endothelial cell differentiation • Facilitate formation of stable tubular structures • Support a flow of perfusate

16. Collaborators

17. References • Martins-Green M, Li Q, Yao M. A New Generation Organ Culture Arising from Cross-Talk Between Primary Human Cell Types. Unpublished. • Nanney, Lillian et al. Unpublished research. • Tang MD et al. Fabrication of collagen gels that contain patterned micrometer-scale cavities. Adv. Mater, 2004.