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NUTRITION AND GROWTH. EVERY LIVING ORGANISM MUST ACQUIRE 2 THINGS FROM ITS ENVIRONMENT IF IT IS TO GROW AND REPRODUCE: STRUCTURAL UNITS ENERGY SOURCE GROUPING ORGANISMS BASED ON NUTRITIONAL REQUIREMENTS: PHOTOHETEROTROPHS PHOTOAUTOTROPHS CHEMOHETEROTROPHS CHEMOAUTOTROPHS.
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EVERY LIVING ORGANISM MUST ACQUIRE 2 THINGS FROM ITS ENVIRONMENT IF IT IS TO GROW AND REPRODUCE: STRUCTURAL UNITS ENERGY SOURCE GROUPING ORGANISMS BASED ON NUTRITIONAL REQUIREMENTS: PHOTOHETEROTROPHS PHOTOAUTOTROPHS CHEMOHETEROTROPHS CHEMOAUTOTROPHS
EXAMPLES OF ORGANISMS FOUND IN EACH GROUP HOW DO ORGANISMS ACQUIRE THEIR NUTRITIONAL NEEDS? IT IS PROVIDED TO THEM IN A MEDIUM MEDIA - LAB OR ENVIRONMENT ALL MEDIA MUST HAVE THE FOLLOWING CHEMICAL ELEMENTS FOR GROWTH - CARBON (C) - NITROGEN (N) - WATER(H2O) MINERALS(S,P,Ca,Mg,Na, etc.)
CARBOHYDRATES ARE SELECTED BECAUSE: WATER SOLUBLE READILY ACCESSIBLE IN LAB AND NATURE USUALLY NON TOXIC IN DILUTE CONCENTRATION EASILY OXIDIZED AND REDUCED, LITTLE MODIFICATION NEEDED
SOME ORGANISMS CAN NOT USE CARBOHYDRATES AS A CARBON SOURCE. THEY WILL USE ALCOHOLS, AMINO ACIDS, FATS, ETC. NITROGEN SOURCES: 1) INORGANIC; 2) ORGANIC INORGANIC SOURCE : NITROGEN GAS(N2) OR NH4CL/(NH4)2SO4 - NITROGEN FIXATION - SYMBIOTIC RELATIONSHIPS.
PROTEIN • BUILDING BLOCKS OF PROTEINS ARE AMINO ACIDS
PRIMARY, SECONDARY,TERTIARY,AND QUATENARY SRUCTURE OF PROTEINS
MINERALS: MICRONUTRIENTS: ZN, CU, CO, MN, MO MACRONUTRIENTS: P, Mg, Ca, S, Na WATER - BIOLOGICAL SOLVENT THAT COMPOSES 80-85% OF THE WEIGHT OF CELLS.
SUBSTANCES WHICH MAY BE ADDED TO MEDIA BUT ARE NOT ALWAYS REQUIRED. - GROWTH FACTORS BUFFERS SOLIDIFYING AGENTS • STERILIZATION OF MEDIA - AUTOCLAVING • FILTRATION a) NEGATIVE b) POSITIVE
CLASSIFICATION OF MEDIA SYNTHETIC OR CHEMICALLY DEFINED MEDIA A CHEMICAL FORMULA CAN BE WRITTEN FOR EVERY INGREDIENT COMPLEX MEDIA A CHEMICAL FORMULA CANNOT BE WRITTEN FOR EVERY INGREDIENT
OXYGEN GAS REQUIREMENTS OF MICROORGANISMS OBLIGATE AEROBES FACULTATIVES AEROTOLERANTS MICROAEROPHILICS OBLIGATE ANAEROBES
GROWTH DEFINITION: INCREASE IN MICROBIAL MASS OR INCREASE IN THE NUMBER OF CELLS GENERATION TIME: THE TIME IT TAKES AN ORGANISM TO REPRODUCE BINARY FISSION SHORTER FOR PROCARYOTES THAN EUCARYOTES ENVIRONMENT EFFECTS GENERATION TIME
WE CAN DEMONSTRATE TYPICAL BACTERIAL GROWTH BY PREPARING A POPULATION GROWTH CURVE. THE BACTERIAL GROWTH CURVE LAG PHASE LOG OR EXPONENTIAL PHASE STATIONARY PHASE DEATH OR DECLINE PHASE