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1 st & 9 th Area Medical Laboratories

1 st & 9 th Area Medical Laboratories. Idaho National Laboratory Interagency Exercise September 2008. Agenda. History AML Team Building Exercise Goals BROOM System Workflow Results Review. Interagency Players. JPEO-CBD EPA DHS DoD NGB CSTs & HAZMAT teams

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1 st & 9 th Area Medical Laboratories

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  1. 1st & 9thArea Medical Laboratories Idaho National Laboratory Interagency Exercise September 2008

  2. Agenda • History • AML Team Building • Exercise Goals • BROOM System • Workflow • Results • Review

  3. Interagency Players • JPEO-CBD • EPA • DHS • DoD • NGB CSTs & HAZMAT teams Also involved in workgroup: - FBI - CDC - NIST

  4. Area Medical Laboratory • INL-1 (‘07) – Five personnel, 9th AML, 4th squad • INL-2 (‘08) – 14 personnel • 9th AML 4th squad – lead • 9th AML 3rd squad • 1st AML 4th squad • 1st AML 3rd squad

  5. Problem “How to prove that a room isn’t contaminated?” • GAO Report – post attack analysis, GAO-05-493T cited lack of validated sampling plans • Evaluation of sampling techniques and their efficacy in an operational environment

  6. Test Plan • Based on INL-1 small scale study – sampling strategies, efficiency of sampling methods, testing methods & validation of dispersal and sampling models – 5 test events • Operational evaluation • Reduce sample numbers without losing robustness • Factors affecting detection minimums

  7. Test Plan (cont.) • Event 1 – ORI • Characterization & Clearance • Event 2 – Characterization & Clearance • Event 3 – Characterization & Clearance • Event 4 – Characterization & Clearance • Event 5 – Characterization & Clearance

  8. Test Facility

  9. Laboratory Set-up Autoclaves Power 3-1 ISO Shelter 4 section TEMPR Storage Grey zone Entry

  10. Sample Collection • Wipes • Swabs • Vacuum socks • DFUs • RMCs

  11. Sample Receipt • Logged into laboratory with the BROOM system

  12. Building Restoration Operations Optimization Model - BROOM • A decision support tool to collect, manage, and analyze sample data • Secure SQL Server database • GIS mapping & 3D visualization • Geostatistical analysis tools • Uncertainty analysis • Interfaces with VSP for statistical sampling design • Data collection • Hand-held wireless PDAs with barcode readers and laser rangefinders • GPS for outdoors • Camera for photo documentation • Paperless data transfer • Secure transmission of data • Chain of custody report WITS – Where Is That Sample?

  13. Extraction & Testing • Samples extracted with PBST • Filter plating • Spiral plating • Q-count • RV-PCR (random 10%)

  14. Results - Characterization

  15. Results - Clearance

  16. Results – RV PCR

  17. Results • Wipe sampling is statistically more effective than vacuum or swab • No statistical difference in effectiveness of recovery between sampling strategies in characterization • RV-PCR vs. culture may be affected by repeat testing • Onsite testing provided rapid feedback to incident commander

  18. Last-Minute RV-PCR Run

  19. AAR • AML successfully deployed/redeployed a tailored team of personnel and equipment • Conducted ISO-9000 equivalent survey of operations • Demonstrated laboratory contamination risk in operational situation • Accurately tracked & reported 99.97% of ~3,000 samples

  20. Review • Reasons for INL-1 & 2 events • Interagency operation • 1st & 9th AML participation • Processes • Results

  21. Acknowledgements • MAJ Brian Walker & SFC Christian Lowry – 9th AML • Dr. Michael Walter – JPEO-CBD • Dr. Robert Knowlton & Mr. Brad Melton – Sandia National Laboratory • Ms. Molly Isbell – Signature Science

  22. Questions???

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