rRNA

1. sdir1-1 sdir1-1/ABI5 1.2 3.2 Col 100 sdir1-1 ABI5 80 sdir1-1/ABF3 11.3 sdir1-1/ABF3 4.1 rRNA 60 Germination (%) sdir1-1/ABF4 14.2 40 sdir1-1/ABF4 20.1 sdir1-1 sdir1-1/ABF3 sdir1-1 /ABI5 1.2 20 11.3 4.1 sdir1-1 /ABI5 3.2 0 ABA (mM) 0 1 2 ABF3 rRNA 1 mM ABA 100 sdir1-1 sdir1-1/ABF4 80 14.2 20.1 60 40 ABF4 20 0 rRNA 2 mM ABA % seedlings after 10 day 100 80 60 abi5-1 abi5-1/SDIR1 40 5.2 11.4 20 0 SDIR1 Col sdir1-1 sdir1-1 /ABI5 1.2 sdir1-1 /ABI5 3.2 sdir1-1/ABF3 4.1 sdir1-1/ABF3 11.3 sdir1-1/ABF4 14.2 sdir1-1/ABF4 20.1 rRNA cotyledon yellow cotyledon green true leaves A B C D

2. Supplemental Figure 4. RNA gel blot analysis of sdir1-1 complementation plants and quantification of the ABA responsive phenotype. Numbers indicate the transgenic lines. RNA gel blot analysis. A. In 35S-ABI5 complementation lines, ABI5 gene expression was detected (upper panel). In 35S-ABF3 complementation lines, ABF3 gene expression was detected (middle panel). In 35S-ABF4 complementation lines, ABF4 gene expression was detected (bottom panel). Ten micrograms of total RNA from each sample was hybridized with gene-specific probes. The rRNA is shown as a loading control. B. ABA dosage-response analysis of germination. Seeds were germinated for 4 days on plates containing different amounts of ABA. The germination percentage without ABA was considered to be 100% and the germination frequency in ABA for these lines was normalized based on this value. Error bars represent SEM (triplicates, n = 40). C. ABA dosage-response analysis of post-germination growth. Results were scored 10 days after plating (triplicates, n = 40). D. RNA gel blot analysis. The 35S-SDIR1 complementation lines in abi5-1. SDIR1 expression was detected.