1 / 18

Ambient Ionization Mass Spectrometry

Ambient Ionization Mass Spectrometry. William Kou Jonathan Mertz. Introduction. The field of Mass Spectrometry using Ambient Ionization techniques has grown exponentially since 2004.

abdalla
Download Presentation

Ambient Ionization Mass Spectrometry

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Ambient Ionization Mass Spectrometry William Kou Jonathan Mertz

  2. Introduction • The field of Mass Spectrometry using Ambient Ionization techniques has grown exponentially since 2004. • The direct analysis approach that these techniques provide allow the collection of samples in their native state with little to no preparation. • These techniques are revolutionizing the field of mass spectrometry and achieving things that were thought to be out of reach.

  3. How It works • The two main techniques used in ambient ionization are known as DESI and DART • These techniques desorb and ionize the analytes so they can be analyzed by the Mass Spectrometer

  4. Desorption • The extraction of an absorbate from an absorbent. • Usually involves a change in state. Ionization • Acquisition of charge by neutral analyte molecules

  5. DESI – Desorption ElectroSpray Ionization • A spray of charged droplets impacts a condensed phase sample on a substrate and creates a thin liquid film • The analyte is then desorbed from the substrate • An additional splash of the solvent creates microdroblets which are then drawn into the inlet of the mass spectrometer. • As the solvent evaporates away, the analyte is ionized.

  6. Advantages • Rapid results • Gentle • Sensitive • Requires little to no sample preparation • Simple, and has potential for portable devices

  7. Signal to noise ratio is much higher in DESI (100:1) than LC-ESI (30:1) • It can detect some compounds that other techniques such as liquid chromatography can not detect. • DESI can be used to examine aqueous solutions on suitable surfaces.

  8. Mass Spectrum using DESI

  9. DART- (Direct Analysis in Real Time) • Shoots a beam of charged metastable gas, such as helium or nitrogen • This beam then collides with the sample, desorbing and charging the sample upon impact • The charged particle is then drawn into the Mass Spectrometer via vacuum

  10. Advantages • Little to no sample preparation • Non-destructive • Continuous sample collection • Rapid Results

  11. Excellent signal to noise ratio

  12. Practical Use • Identification of traces left behind by explosives, toxic industrial compounds, chemical warfare agents, illicit drugs, some foods, inks, fingerprints and skin. - This can provide valid evidence to convict someone in court. • Can be used by pharmaceutical companies to rapidly analyze compounds in their natural form

  13. Able to analyze illicit drugs within biological fluids - this is valuable because most other methods require sample extraction before analysis • Identifying contaminants in foods such as melamine, which can make the product appear to have a higher protein content. • DESI can be used to produce images of latent fingerprints

  14. Analysis of ink can be performed by DART, picking up differences in inks. • An application for this could be to verify that documents have not been changed

  15. Conclusion Further growth in the field and applications of these techniques is ongoing. The success in the examples given show that these techniques are promising and worth further investigation and testing.

  16. Disadvantages of these techniques • There is a certain amount of “noise” present in the spectrum - to remedy this problem you could easily use tandem MS or MS/MS

  17. These two techniques do not readily ionize every compound you may come across - there are techniques which were developed from DESI and DART (listed in the article) that can account for these compounds. - The use of different dopants also effects the detection of desired analytes - There are also more techniques and dopants being developed and discovered

  18. http://www.youtube.com/watch?v=SJk9OCNmB2c

More Related