40 likes | 178 Views
2010.02.23. SCHDGP005 PCR report. 1. Primer list. 2010.02.23. SCHDGP005 PCR report. 2. The Result of PCR. 2-2. PCR Condition. 2-1. PCR reaction combination. 2010.02.23. SCHDGP005 PCR report. 2-4. Electrophoresis : primer set 1,2,3 _ 추가 PCR. 2-3. Electrophoresis. 1kb Ladder.
E N D
2010.02.23 SCHDGP005 PCR report 1. Primer list
2010.02.23 SCHDGP005 PCR report 2. The Result of PCR 2-2. PCR Condition 2-1. PCR reaction combination
2010.02.23 SCHDGP005 PCR report 2-4. Electrophoresis : primer set 1,2,3 _추가 PCR 2-3. Electrophoresis 1kb Ladder Primer set Primer set 1 2 3 1 2 3 4 5 6 7 1.5% agarose gel 1.5% agarose gel 3. Sequencing :Primer set 1, 4~7 PCR product를 gel elution하여 양방향 sequencing
2010.02.23 SCHDGP005 PCR report 4. PCR 추가 : template 농도 변경 4-3. Electrophoresis 4-1. PCR reaction combination Primer set 1 2 3 1% agarose gel 4-2. PCR Condition ; 이전과 동일 5. Sequencing :2,3번 primer set는 sequencing 못함