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Reprogramming of somatic cells via TAT- mediated protein transduction of recombinant factors

Reprogramming of somatic cells via TAT- mediated protein transduction of recombinant factors. Presented by Wu Meng. Induced Pluripotent Stem Cells. Safe. Virus 2006. Plasmids 2007. Proteins 2009. mRNA 2010. Transduction. Protein Transduction Domain. PTD -PTD. Construct.

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Reprogramming of somatic cells via TAT- mediated protein transduction of recombinant factors

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  1. Reprogramming of somatic cellsvia TAT- mediated protein transduction of recombinant factors Presented by Wu Meng

  2. Induced Pluripotent Stem Cells

  3. Safe Virus 2006 Plasmids 2007 Proteins 2009 mRNA 2010

  4. Transduction Protein Transduction Domain PTD-PTD Construct Target gene PTD-target gene fusion protein Expression Add to cell culture medium Injection

  5. PTD Mediated Protein Delivery PTD PTD

  6. TAT: Powerful in vivo protein transduction system

  7. TAT: Powerful in vivo protein transduction system HIV-1 TAT PTD Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg Fluorescence confocal microscopy of hemispheric sagittal brain sections (top) and skeletal muscle tissue sections (bottom) isolated from mice 20 min after ip injection. Steven F. Dowdy Science ,1999

  8. Characterization of TAT-mediated transduction of RFs

  9. TAT PTD and 11R mediated RF transduction

  10. Transduced TAT-RFs and 11R-RFs are transcriptionally active

  11. TAT-Sox2 (nM) 25 50 75 Virus O/K/M TAT-RFs are functionally active in reprogramming(Mouse NPC)

  12. TAT-RFs are functionally active in reprogramming(Human HFFs)

  13. Reprogramming of human foreskin fibroblast cells using recombinant TAT-RFs.

  14. Significiency Detailed characterization of recombinant RFs in reprogramming Quicker process and higher efficiency than current protocl To facilitate the generation of clinically useful and genetic material-free human iPSCs.

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