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This study explores the potential of TAT-mediated protein transduction in reprogramming somatic cells to induced pluripotent stem cells (iPSCs). The use of TAT fusion proteins successfully delivers recombinant factors for efficient iPSC generation, offering a safer and faster alternative to current protocols. The findings provide significant insights into the development of clinically useful and genetic material-free human iPSCs.
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Reprogramming of somatic cellsvia TAT- mediated protein transduction of recombinant factors Presented by Wu Meng
Safe Virus 2006 Plasmids 2007 Proteins 2009 mRNA 2010
Transduction Protein Transduction Domain PTD-PTD Construct Target gene PTD-target gene fusion protein Expression Add to cell culture medium Injection
PTD Mediated Protein Delivery PTD PTD
TAT: Powerful in vivo protein transduction system
TAT: Powerful in vivo protein transduction system HIV-1 TAT PTD Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg Fluorescence confocal microscopy of hemispheric sagittal brain sections (top) and skeletal muscle tissue sections (bottom) isolated from mice 20 min after ip injection. Steven F. Dowdy Science ,1999
TAT-Sox2 (nM) 25 50 75 Virus O/K/M TAT-RFs are functionally active in reprogramming(Mouse NPC)
TAT-RFs are functionally active in reprogramming(Human HFFs)
Reprogramming of human foreskin fibroblast cells using recombinant TAT-RFs.
Significiency Detailed characterization of recombinant RFs in reprogramming Quicker process and higher efficiency than current protocl To facilitate the generation of clinically useful and genetic material-free human iPSCs.